Zeta Potential and Electrophoretic Investigation of Ion Channel Regulation and Membrane Characteristics in Cystic Fibrosis

Abstract

Cystic Fibrosis (CF) is a genetic disease that causes mutations in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) channel, which is responsible for transferring Cl- outside the cell, and CF affects its gating properties. These mutations disrupt the distribution of Cl- ions across the cell membrane, leading to a reduced attraction of water molecules outside the cell. In laboratory settings, electrophysiological methods are widely employed to diagnose mutations or demonstrate the effectiveness of specific therapeutics in improving channel function. However, these methods are complex, require strong expertise, and demand specific culturing conditions. The Zeta potential and dielectrophoresis analyzes are two techniques that are mainly representative of ions concentration around the cell, despite their potential relevance, their role in Cystic Fibrosis research has not yet been explored. Therefore, this project has successfully employed these two simple and easily reproducible techniques in differentiating between cells under various states of activation and inhibition of membrane channels, as well as expanded the gap of limited electrophysiological options for Cystic Fibrosis research.

Date of Award	14 May 2024
Original language	American English
Supervisor	Michael Hughes (Supervisor)