Bio-guided fractionation of Tamarix nilotica aqueous extract based on its anticancer property

  • Maryam K. Al Kaabi

Student thesis: Master's Thesis

Abstract

The UAE hosts a flora that displays remarkable adaptation to arid weather conditions. Phytochemicals found in such arid plants are widely researched as potential pharmaceutical and nutraceutical products. In this study, phytochemicals present in two UAE native plant species, Arthrocnemum macrostachyum and Tamarix nilotica were extracted in water using two methods (maceration and microwave assisted extraction (MAE)) and subsequently evaluated for their in vitro cytotoxicity (based on LD50 in μg/mL) towards three cancer cell lines; breast (MDA-MB-231), colon (HCT-116) and lung (A-549). Results suggest that (1) MAE is more efficient compared to maceration for the recovery of metabolites from plant biomass based on measurements of total phenolic content, radical scavenging activity and bioactivity of extracts based on in vitro cytotoxicity (2) only T. nilotica extracts were found to be bioactive based on cytotoxicity measurements (LD50 of crude extract < 1000 μg/mL), and (3) cancer cell lines exhibited differential sensitivity to T. nilotica crude extracts, with breast cancer cells being the most sensitive and lung cancer cells being the least sensitive. Solid phase fractionation of T. nilotica crude extract in 0 to 100% methanol yielded several fractions that were 100-fold more cytotoxic compared to crude unfractionated extract. In particular, the 30% and 70% methanol fractions exhibited the highest cytotoxicity (LD50
Date of AwardMay 2020
Original languageAmerican English

Keywords

  • Halophytes
  • Tamarix nilotica
  • Arthrocnemum macrostachyum
  • MAE
  • Antitumor
  • Antioxidant
  • Bio-guided fractionation.

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