TY - JOUR
T1 - Zein-based oral drug delivery system targeting activated macrophages
AU - Lee, Sungmun
AU - Alwahab, Noaf Salah Ali
AU - Moazzam, Zainab Muhammad
N1 - Funding Information:
This project was supported by the Khalifa University Internal Research Fund (KUIRF) level I ( 2002-21020A ). Parts of experiments were carried out in Larry McIntire's laboratory at Georgia Institute of Technology. The authors would like to thank Larry McIntire (Ph.D. The Wallace H. Coulter Chair & Professor), Suzanne Eskin (Ph.D. Principal Research Scientist), and Lisa Schildmeyer (Ph.D. Senior Research Scientist) for their support and valuable comments. The authors took SEM images using a SEM in Masdar Institute of Science and Technology and the authors also thank Masdar Institute for the SEM.
PY - 2013
Y1 - 2013
N2 - Reactive oxygen species (ROS) play an important role in the pathogenesis of rheumatoid arthritis (RA). ROS such as hydrogen peroxide and superoxide are overproduced by activated macrophages in RA. As scavengers of ROS, enzymatic proteins such as catalase and superoxide dismutase (SOD) have a great therapeutic potential; however, in vivo application is limited especially when they are orally administered. Although, the oral route is the most convenient for drug administration, therapeutic proteins are easily degraded in vivo by the harsh conditions of gastrointestinal (GI) tract. Here, we introduce a novel drug delivery system composed of zein, a plant storage protein derived from maize. We demonstrate that zein nanoparticles can protect therapeutic proteins, catalase and SOD, from the harsh conditions of GI tract. Folate-conjugated catalase or SOD in zein nanoparticles can target the activated macrophages and scavenge the ROS generated by macrophages in vitro. This novel drug delivery system will be applicable to other orally administered treatments based on the protective property in the harsh conditions of GI tract.
AB - Reactive oxygen species (ROS) play an important role in the pathogenesis of rheumatoid arthritis (RA). ROS such as hydrogen peroxide and superoxide are overproduced by activated macrophages in RA. As scavengers of ROS, enzymatic proteins such as catalase and superoxide dismutase (SOD) have a great therapeutic potential; however, in vivo application is limited especially when they are orally administered. Although, the oral route is the most convenient for drug administration, therapeutic proteins are easily degraded in vivo by the harsh conditions of gastrointestinal (GI) tract. Here, we introduce a novel drug delivery system composed of zein, a plant storage protein derived from maize. We demonstrate that zein nanoparticles can protect therapeutic proteins, catalase and SOD, from the harsh conditions of GI tract. Folate-conjugated catalase or SOD in zein nanoparticles can target the activated macrophages and scavenge the ROS generated by macrophages in vitro. This novel drug delivery system will be applicable to other orally administered treatments based on the protective property in the harsh conditions of GI tract.
KW - Inflammatory disease
KW - Oral drug delivery
KW - Reactive oxygen species
KW - Zein
UR - http://www.scopus.com/inward/record.url?scp=84884142101&partnerID=8YFLogxK
U2 - 10.1016/j.ijpharm.2013.07.026
DO - 10.1016/j.ijpharm.2013.07.026
M3 - Article
AN - SCOPUS:84884142101
SN - 0378-5173
VL - 454
SP - 388
EP - 393
JO - International Journal of Pharmaceutics
JF - International Journal of Pharmaceutics
IS - 1
ER -