Viscoelastic properties of human mesenchymal stem cells

Wenxiao Pan, Erik Petersen, Ning Cai, Gang Ma, Jian Run Lee, Zhiqin Feng, Kin Liao, Kam W. Leong

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

21 Scopus citations

Abstract

In this study, we investigated the viscoelasticity of individual bone marrow-derived adult human Mesenchymal Stem Cells (hMSCs), and the role of specific cytoskeletal component-F-actin microfilaments on the mechanical properties of individual hMSCs. The mechanical properties of hMSCs were determined using the micropipette aspiration technique coupled with a viscoelastic solid model of the cell. For the hMSCs under control conditions the instantaneous Young's modulus E0 was found to be 886±289(Pa), the equilibrium Young's modulus E 372±125(Pa), and the apparent viscosity μ, 2714±1626(Pa·S). After exposed to 2μM of chemical agent-cytochalasin D that disrupt the F-actin microfilaments, the Young's moduli of hMSCs decreased by up to 72% and the apparent viscosity increased by 167%. These findings suggest that microfilaments are crucial in providing the viscoelastic properties of the hMSCs, and changes in the structure and properties of them may influence the mechanical properties of hMSCs significantly.

Original languageBritish English
Title of host publicationProceedings of the 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
Pages4854-4857
Number of pages4
StatePublished - 2005
Event2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005 - Shanghai, China
Duration: 1 Sep 20054 Sep 2005

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume7 VOLS
ISSN (Print)0589-1019

Conference

Conference2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
Country/TerritoryChina
CityShanghai
Period1/09/054/09/05

Keywords

  • Actin
  • Apparent viscosity
  • Equilibrium young's modulus
  • Human Mesenchymal Stem Cells (hMSCs)
  • Instantaneous young's modulus
  • Mechanical properties
  • Micropipette aspiration
  • Viscoelasticity

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