VEGF-B prevents excessive angiogenesis by inhibiting FGF2/FGFR1 pathway

Chunsik Lee, Rongyuan Chen, Guangli Sun, Xialin Liu, Xianchai Lin, Chang He, Liying Xing, Lixian Liu, Lasse Dahl Ejby Jensen, Anil Kumar, Harald F. Langer, Xiangrong Ren, Jianing Zhang, Lijuan Huang, Xiangke Yin, Jongkyong Kim, Juanhua Zhu, Guanqun Huang, Jiani Li, Weiwei LuWei Chen, Juanxi Liu, Jiaxin Hu, Qihang Sun, Weisi Lu, Lekun Fang, Shasha Wang, Haiqing Kuang, Yihan Zhang, Geng Tian, Jia Mi, Bi Ang Kang, Masashi Narazaki, Aaron Prodeus, Luc Schoonjans, David M. Ornitz, Jean Gariepy, Guy Eelen, Mieke A. Dewerchin, Yunlong Yang, Jingsong Ou, Antonio Mora, Jin Yao, Chen Zhao, Yizhi Liu, Peter F.M. Carmeliet, Yihai Cao, Xuri Li

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Although VEGF-B was discovered as a VEGF-A homolog a long time ago, the angiogenic effect of VEGF-B remains poorly understood with limited and diverse findings from different groups. Notwithstanding, drugs that inhibit VEGF-B together with other VEGF family members are being used to treat patients with various neovascular diseases. It is therefore critical to have a better understanding of the angiogenic effect of VEGF-B and the underlying mechanisms. Using comprehensive in vitro and in vivo methods and models, we reveal here for the first time an unexpected and surprising function of VEGF-B as an endogenous inhibitor of angiogenesis by inhibiting the FGF2/FGFR1 pathway when the latter is abundantly expressed. Mechanistically, we unveil that VEGF-B binds to FGFR1, induces FGFR1/VEGFR1 complex formation, and suppresses FGF2-induced Erk activation, and inhibits FGF2-driven angiogenesis and tumor growth. Our work uncovers a previously unrecognized novel function of VEGF-B in tethering the FGF2/FGFR1 pathway. Given the anti-angiogenic nature of VEGF-B under conditions of high FGF2/FGFR1 levels, caution is warranted when modulating VEGF-B activity to treat neovascular diseases. © 2023, West China Hospital, Sichuan University.
Original languageAmerican English
JournalSignal Transduction and Targeted Therapy
Volume8
Issue number1
DOIs
StatePublished - 2023

Keywords

  • fibroblast growth factor 2
  • fibroblast growth factor receptor 1
  • vasculotropin
  • FGFR1 protein
  • human
  • vasculotropin B
  • angiogenesis
  • animal cell
  • animal experiment
  • animal model
  • Article
  • cell culture
  • cell migration
  • chemoluminescence
  • enzyme linked immunosorbent assay
  • high performance liquid chromatography
  • human cell
  • immunofluorescence
  • immunoprecipitation
  • immunoreactivity
  • mass spectrometry
  • mouse
  • neovascular glaucoma
  • nonhuman
  • phosphorylation
  • protein determination
  • tumor growth
  • umbilical vein endothelial cell
  • Western blotting
  • genetics
  • immunotherapy
  • Fibroblast Growth Factor 2
  • Humans
  • Immunotherapy
  • Receptor
  • Fibroblast Growth Factor
  • Type 1
  • Vascular Endothelial Growth Factor B

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