Using an innovative pH-stat CO2 feeding strategy to enhance cell growth and C-phycocyanin production from Spirulina platensis

Chun Yen Chen, Pei Chun Kao, Chung Hong Tan, Pau Loke Show, Wai Yan Cheah, Wen Lung Lee, Tau Chuan Ling, Jo Shu Chang

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Spirulina platensis can mitigate CO2 emissions and simultaneously produce an antioxidant called C-phycocyanin (C-PC), which is a high value nutraceutical product. An innovative pH control system was used to cultivate S. platensis, in which CO2 feeding, instead of acid/alkaline titration, was used to control the pH of the culture. The optimum culture pH was 9.5. Compared to continuous CO2 feeding system, the pH control system improved the CO2 removal efficiency from 13.6% to 62.3%. The C-PC content and productivity were also enhanced to 16.8% and 0.17 g/L/d, respectively. Therefore, the proposed pH control system is economic and sustainable as it avoids the use of acid/alkaline and reduces the overall CO2 emissions. A two-stage C-PC purification process combining fractional precipitation and ion exchange chromatography could achieve the highest purity (A615/A280) of 4.33 with a 33% C-PC recovery.

Original languageBritish English
Pages (from-to)78-85
Number of pages8
JournalBiochemical Engineering Journal
Volume112
DOIs
StatePublished - 15 Aug 2016

Keywords

  • C-Phycocyanin
  • Chromatography
  • Microalgae
  • Optimization
  • PH control system
  • Purification

Fingerprint

Dive into the research topics of 'Using an innovative pH-stat CO2 feeding strategy to enhance cell growth and C-phycocyanin production from Spirulina platensis'. Together they form a unique fingerprint.

Cite this