Unraveling microbial community by next-generation sequencing in living membrane bioreactors for wastewater treatment

Carlo Cabreros, Mary Vermi Aizza Corpuz, Fabiano Castrogiovanni, Laura Borea, Anna Sandionigi, Giovanni Vigliotta, Florencio Ballesteros, Sebastià Puig, Shadi W. Hasan, Gregory V. Korshin, Vincenzo Belgiorno, Antonio Buonerba, Vincenzo Naddeo

    Research output: Contribution to journalArticlepeer-review

    3 Scopus citations

    Abstract

    This study delves into the microbial community complexity and its role in self-forming dynamic membrane (SFDM) systems, designed to remove nutrients and pollutants from wastewater, by means of the analysis of Next-Generation Sequencing (NGS) data. In these systems, microorganisms are naturally incorporated into the SFDM layer, which acts as a biological and physical filter. The microorganisms present in an innovative and highly efficient aerobic, electrochemically enhanced, encapsulated SFDM bioreactor were studied to elucidate the nature of the dominant microbial communities present in sludge and in encapsulated SFDM, patented as living membrane® (LM) of the experimental setup. The results were compared to those obtained from the microbial communities found in similar experimental reactors without an applied electric field. The data gathered from the NGS microbiome profiling showed that the microbial consortia found in the experimental systems are comprised of archaeal, bacterial, and fungal communities. However, the distribution of the microbial communities found in e-LMBR and LMBR had significant differences. The results showed that the presence of an intermittently applied electric field in e-LMBR promotes the growth of some types of microorganisms (mainly electroactive microorganisms) responsible for the highly efficient treatment of the wastewater and for the mitigation of the membrane fouling found for those bioreactors.

    Original languageBritish English
    Article number163965
    JournalScience of the Total Environment
    Volume886
    DOIs
    StatePublished - 15 Aug 2023

    Keywords

    • Electro-encapsulated living membrane bioreactor
    • Electrochemical process
    • Encapsulated living membrane
    • Fouling mitigation
    • Microbial growth

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