Two-dimensional electrophoresis analysis of glutathione affinity-selected proteins from the clam Tapes semidecussatus: Evidence for tissue-specific expression of redox proteins

V. Dowling; B. McDonagh; E. M. Cotter; N. O'Brien; F. van Pelt; J. O'Halloran; D. Sheehan

doi:10.1016/j.cbd.2006.01.002

Two-dimensional electrophoresis analysis of glutathione affinity-selected proteins from the clam Tapes semidecussatus: Evidence for tissue-specific expression of redox proteins

V. Dowling, B. McDonagh, E. M. Cotter, N. O'Brien, F. van Pelt, J. O'Halloran, D. Sheehan

Chemistry

University College Cork

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Proteins from gill, digestive gland and mantle of the clam Tapes semidecussatus were selected on glutathione (GSH) agarose to simplify proteomic comparison. Analysis by two-dimensional gel electrophoresis (2D SDS PAGE) revealed tissue-specific patterns of protein expression with some spots common to all tissues. Immunoblotting of gill and digestive gland separations identified some spots as glutathione transferases (GSTs). In gill and digestive gland several spots were immunoblotted with Pi class GSTs indicating multiple isoenzymes. Selected spots were excised, digested with trypsin and analyzed by reversed phase C-18 high performance liquid chromatography and tandem mass spectrometry. This confirmed that gill and digestive gland share some GST isoenzymes. Our results suggest that this clam expresses a complex tissue-specific pattern of GSH-binding proteins, which may reflect different redox requirements in each tissue.

Original language	British English
Pages (from-to)	267-272
Number of pages	6
Journal	Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume	1
Issue number	3
DOIs	https://doi.org/10.1016/j.cbd.2006.01.002
State	Published - Sep 2006

Keywords

Affinity chromatography
Ecotoxicology
Glutathione transferase
Proteomics
Redox status
Tapes semidecussatus

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10.1016/j.cbd.2006.01.002

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Dowling, V., McDonagh, B., Cotter, E. M., O'Brien, N., van Pelt, F., O'Halloran, J., & Sheehan, D. (2006). Two-dimensional electrophoresis analysis of glutathione affinity-selected proteins from the clam Tapes semidecussatus: Evidence for tissue-specific expression of redox proteins. Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, 1(3), 267-272. https://doi.org/10.1016/j.cbd.2006.01.002

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title = "Two-dimensional electrophoresis analysis of glutathione affinity-selected proteins from the clam Tapes semidecussatus: Evidence for tissue-specific expression of redox proteins",

abstract = "Proteins from gill, digestive gland and mantle of the clam Tapes semidecussatus were selected on glutathione (GSH) agarose to simplify proteomic comparison. Analysis by two-dimensional gel electrophoresis (2D SDS PAGE) revealed tissue-specific patterns of protein expression with some spots common to all tissues. Immunoblotting of gill and digestive gland separations identified some spots as glutathione transferases (GSTs). In gill and digestive gland several spots were immunoblotted with Pi class GSTs indicating multiple isoenzymes. Selected spots were excised, digested with trypsin and analyzed by reversed phase C-18 high performance liquid chromatography and tandem mass spectrometry. This confirmed that gill and digestive gland share some GST isoenzymes. Our results suggest that this clam expresses a complex tissue-specific pattern of GSH-binding proteins, which may reflect different redox requirements in each tissue.",

keywords = "Affinity chromatography, Ecotoxicology, Glutathione transferase, Proteomics, Redox status, Tapes semidecussatus",

author = "V. Dowling and B. McDonagh and Cotter, {E. M.} and N. O'Brien and {van Pelt}, F. and J. O'Halloran and D. Sheehan",

year = "2006",

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doi = "10.1016/j.cbd.2006.01.002",

language = "British English",

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Dowling, V, McDonagh, B, Cotter, EM, O'Brien, N, van Pelt, F, O'Halloran, J & Sheehan, D 2006, 'Two-dimensional electrophoresis analysis of glutathione affinity-selected proteins from the clam Tapes semidecussatus: Evidence for tissue-specific expression of redox proteins', Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, vol. 1, no. 3, pp. 267-272. https://doi.org/10.1016/j.cbd.2006.01.002

Two-dimensional electrophoresis analysis of glutathione affinity-selected proteins from the clam Tapes semidecussatus: Evidence for tissue-specific expression of redox proteins. / Dowling, V.; McDonagh, B.; Cotter, E. M. et al.
In: Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, Vol. 1, No. 3, 09.2006, p. 267-272.

Research output: Contribution to journal › Article › peer-review

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T1 - Two-dimensional electrophoresis analysis of glutathione affinity-selected proteins from the clam Tapes semidecussatus

T2 - Evidence for tissue-specific expression of redox proteins

AU - Dowling, V.

AU - McDonagh, B.

AU - Cotter, E. M.

AU - O'Brien, N.

AU - van Pelt, F.

AU - O'Halloran, J.

AU - Sheehan, D.

PY - 2006/9

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AB - Proteins from gill, digestive gland and mantle of the clam Tapes semidecussatus were selected on glutathione (GSH) agarose to simplify proteomic comparison. Analysis by two-dimensional gel electrophoresis (2D SDS PAGE) revealed tissue-specific patterns of protein expression with some spots common to all tissues. Immunoblotting of gill and digestive gland separations identified some spots as glutathione transferases (GSTs). In gill and digestive gland several spots were immunoblotted with Pi class GSTs indicating multiple isoenzymes. Selected spots were excised, digested with trypsin and analyzed by reversed phase C-18 high performance liquid chromatography and tandem mass spectrometry. This confirmed that gill and digestive gland share some GST isoenzymes. Our results suggest that this clam expresses a complex tissue-specific pattern of GSH-binding proteins, which may reflect different redox requirements in each tissue.

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KW - Ecotoxicology

KW - Glutathione transferase

KW - Proteomics

KW - Redox status

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Dowling V, McDonagh B, Cotter EM, O'Brien N, van Pelt F, O'Halloran J et al. Two-dimensional electrophoresis analysis of glutathione affinity-selected proteins from the clam Tapes semidecussatus: Evidence for tissue-specific expression of redox proteins. Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics. 2006 Sep;1(3):267-272. doi: 10.1016/j.cbd.2006.01.002

Two-dimensional electrophoresis analysis of glutathione affinity-selected proteins from the clam Tapes semidecussatus: Evidence for tissue-specific expression of redox proteins

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