TY - JOUR
T1 - Transcriptomic plasticity of the hypothalamic osmoregulatory control centre of the Arabian dromedary camel
AU - Lin, Panjiao
AU - Gillard, Benjamin T.
AU - Pauža, Audrys G.
AU - Iraizoz, Fernando A.
AU - Ali, Mahmoud A.
AU - Mecawi, Andre S.
AU - Alim, Fatma Z.Djazouli
AU - Romanova, Elena V.
AU - Burger, Pamela A.
AU - Greenwood, Michael P.
AU - Adem, Abdu
AU - Murphy, David
N1 - Funding Information:
This research was supported by grants from the Leverhulme Trust (RPG-2017-287) to B.T.G., F.A.I., D.M. and M.P.G., the Biotechnology and Biological Sciences Research Council (BBSRC; BB/R016879/1) to D.M. and M.P.G., the United Arab Emirates University (UAEU)-Program for Advanced Research (UPAR-31M242) to A.A., the São Paulo Research Foundation (FAPESP, 2019/27581-0) to A.S.M., and the UIUC NIDA Centre for Neuroproteomics (P30 DA018310) to E.V.R. Students were supported by grants from the Biotechnology and Biological Sciences Research Council-SWBio DTP programme (BBSRC; BB/M009122/1) to B.T.G. and the British Heart Foundation (BHF; FS/17/60/33474) to A.G.P. We thank Professor José Antunes-Rodrigues (School of Medicine, Ribeirão Preto, São Paulo, Brazil) for the use of laboratory facilities to measure ANG II.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Water conservation is vital for life in the desert. The dromedary camel (Camelus dromedarius) produces low volumes of highly concentrated urine, more so when water is scarce, to conserve body water. Two hormones, arginine vasopressin and oxytocin, both produced in the supraoptic nucleus, the core hypothalamic osmoregulatory control centre, are vital for this adaptive process, but the mechanisms that enable the camel supraoptic nucleus to cope with osmotic stress are not known. To investigate the central control of water homeostasis in the camel, we first build three dimensional models of the camel supraoptic nucleus based on the expression of the vasopressin and oxytocin mRNAs in order to facilitate sampling. We then compare the transcriptomes of the supraoptic nucleus under control and water deprived conditions and identified genes that change in expression due to hyperosmotic stress. By comparing camel and rat datasets, we have identified common elements of the water deprivation transcriptomic response network, as well as elements, such as extracellular matrix remodelling and upregulation of angiotensinogen expression, that appear to be unique to the dromedary camel and that may be essential adaptations necessary for life in the desert.
AB - Water conservation is vital for life in the desert. The dromedary camel (Camelus dromedarius) produces low volumes of highly concentrated urine, more so when water is scarce, to conserve body water. Two hormones, arginine vasopressin and oxytocin, both produced in the supraoptic nucleus, the core hypothalamic osmoregulatory control centre, are vital for this adaptive process, but the mechanisms that enable the camel supraoptic nucleus to cope with osmotic stress are not known. To investigate the central control of water homeostasis in the camel, we first build three dimensional models of the camel supraoptic nucleus based on the expression of the vasopressin and oxytocin mRNAs in order to facilitate sampling. We then compare the transcriptomes of the supraoptic nucleus under control and water deprived conditions and identified genes that change in expression due to hyperosmotic stress. By comparing camel and rat datasets, we have identified common elements of the water deprivation transcriptomic response network, as well as elements, such as extracellular matrix remodelling and upregulation of angiotensinogen expression, that appear to be unique to the dromedary camel and that may be essential adaptations necessary for life in the desert.
UR - http://www.scopus.com/inward/record.url?scp=85138458720&partnerID=8YFLogxK
U2 - 10.1038/s42003-022-03857-0
DO - 10.1038/s42003-022-03857-0
M3 - Article
C2 - 36151304
AN - SCOPUS:85138458720
SN - 2399-3642
VL - 5
JO - Communications Biology
JF - Communications Biology
IS - 1
M1 - 1008
ER -