Toxins from the venom of the green mamba Dendroaspis angusticeps that inhibit the binding of quinuclidinyl benzilate to muscarinic acetylcholine receptors

Abdu Adem, Anette Åsblom, Gunnar Johansson, Paul M. Mbugua, Evert Karlsson

Research output: Contribution to journalArticlepeer-review

90 Scopus citations

Abstract

Two protein toxins that displace the muscarinic antagonist quinuclidinyl benzilate from rat cortex synaptosomal membranes have been isolated from the green mamba (Dendroaspis angusticeps) venom by gel filtration on sephadex G-50, chromatography on the ion-exchangers Bio-Rex 70 and Sulphopropyl-Sephadex C-25 and reversed-phase HPLC. Toxin 1 has 64 amino acids and four disulfides and a formula weight of 7200 and the corresponding values for toxin 2 are 63, 4 and 6840, respectively. Ultracentrifugation gave a molecular weight of 6900 for toxin 1 and 6700 for toxin 2, Quinuclidinyl benzilate that binds to all types of muscarinic cholinergic receptor was displaced to about 50% by both toxins. This partial displacement indicates that the toxins might be specific for one subtype of receptor.

Original languageBritish English
Pages (from-to)340-345
Number of pages6
JournalBBA - Molecular Cell Research
Volume968
Issue number3
DOIs
StatePublished - 11 Mar 1988

Keywords

  • (Green mamba)
  • Ligand binding
  • Muscarinic cholinergic receptor
  • Quinuclidinyl benzilate
  • Snake toxin

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