TY - JOUR
T1 - Total levels, localization patterns, and proportions of sperm exhibiting phospholipase C zeta are significantly correlated with fertilization rates after intracytoplasmic sperm injection
AU - Yelumalai, Suseela
AU - Yeste, Marc
AU - Jones, Celine
AU - Amdani, Siti N.
AU - Kashir, Junaid
AU - Mounce, Ginny
AU - Da Silva, Sarah J.Martins
AU - Barratt, Christopher L.
AU - McVeigh, Enda
AU - Coward, Kevin
N1 - Funding Information:
The research described herein was funded by project grants, awarded to K.C., from The Royal Society (UK) and the Oxford University Medical Research Fund. S.Y. was funded by the Ministry of Higher Education (MOHE), Malaysia, and the Nuffield Department of Obstetrics and Gynaecology , University of Oxford. M.Y. was funded by the European Commission , FP7-People Programme, Marie Curie-IEF (Intra-European Fellowships) (Grant Number: 626061 ). S.N.A. was funded by a Chancellor's Scholarship from the Universiti Brunei Darussalam.
Publisher Copyright:
© 2015 American Society for Reproductive Medicine.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - Objective To study the relationship of total levels, localization patterns, and proportions of sperm exhibiting phospholipase C zeta, with fertilization rates after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Design Laboratory study; controls vs. patients after IVF (n = 27) or ICSI (n = 17) treatment. Setting Fertility center. Patient(s) A total of 44 semen samples, subjected to either IVF or ICSI treatment. Oocyte collection, ICSI or IVF, determination of sperm concentration and motility, and immunocytochemical analyses of phospholipase C zeta (PLCζ). Intervention(s) None. Main Outcome Measure(s) Percentages of sperm exhibiting PLCζ. Result(s) Significant positive correlation between ICSI fertilization rates and total levels, localization patterns, and the proportion (percentage) of sperm exhibiting PLCζ. Total levels, localization patterns, and the proportion of sperm exhibiting PLCζ are correlated with fertilization rates for ICSI, but not for IVF. Conclusion(s) Evaluating total levels, localization patterns, and proportions of PLCζ may represent a useful diagnostic tool for clinical purposes in men for whom IVF is not advised or has previously failed. This clinical study further supports the fundamental role of PLCζ in the oocyte activation process.
AB - Objective To study the relationship of total levels, localization patterns, and proportions of sperm exhibiting phospholipase C zeta, with fertilization rates after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Design Laboratory study; controls vs. patients after IVF (n = 27) or ICSI (n = 17) treatment. Setting Fertility center. Patient(s) A total of 44 semen samples, subjected to either IVF or ICSI treatment. Oocyte collection, ICSI or IVF, determination of sperm concentration and motility, and immunocytochemical analyses of phospholipase C zeta (PLCζ). Intervention(s) None. Main Outcome Measure(s) Percentages of sperm exhibiting PLCζ. Result(s) Significant positive correlation between ICSI fertilization rates and total levels, localization patterns, and the proportion (percentage) of sperm exhibiting PLCζ. Total levels, localization patterns, and the proportion of sperm exhibiting PLCζ are correlated with fertilization rates for ICSI, but not for IVF. Conclusion(s) Evaluating total levels, localization patterns, and proportions of PLCζ may represent a useful diagnostic tool for clinical purposes in men for whom IVF is not advised or has previously failed. This clinical study further supports the fundamental role of PLCζ in the oocyte activation process.
KW - In vitro fertilization
KW - intracytoplasmic sperm injection
KW - oocyte activation
KW - phospholipase C zeta
KW - total fertilization failure
UR - http://www.scopus.com/inward/record.url?scp=84940718062&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2015.05.018
DO - 10.1016/j.fertnstert.2015.05.018
M3 - Article
C2 - 26054556
AN - SCOPUS:84940718062
SN - 0015-0282
VL - 104
SP - 561-568.e4
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 3
ER -