Thermoseparating aqueous two-phase systems: Recent trends and mechanisms

Yoong Kit Leong, John Chi Wei Lan, Hwei San Loh, Tau Chuan Ling, Chien Wei Ooi, Pau Loke Show

Research output: Contribution to journalReview articlepeer-review

25 Scopus citations


Having the benefits of being environmentally friendly, providing a mild environment for bioseparation, and scalability, aqueous two-phase systems (ATPSs) have increasingly caught the attention of industry and researchers for their application in the isolation and recovery of bioproducts. The limitations of conventional ATPSs give rise to the development of temperature-induced ATPSs that have distinctive thermoseparating properties and easy recyclability. This review starts with a brief introduction to thermoseparating ATPSs, including its history, unique characteristics and advantages, and lastly, key factors that influence partitioning. The underlying mechanism of temperature-induced ATPSs is covered together with a summary of recent applications. Thermoseparating ATPSs have been proven as a solution to the demand for economically favorable and environmentally friendly industrial-scale bioextraction and purification techniques.

Original languageBritish English
Pages (from-to)640-647
Number of pages8
JournalJournal of Separation Science
Issue number4
StatePublished - 1 Feb 2016


  • Aqueous two-phase systems
  • Bioseparation
  • Downstream processing
  • Thermoseparating polymers


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