TY - JOUR
T1 - Synthesis of one-dimensional magnetite hydroxyapatite nanorods on reduced graphene oxide sheets for selective separation and controlled delivery of hemoglobin
AU - Bharath, G.
AU - Rambabu, K.
AU - Hai, Abdul
AU - Anwer, Shoaib
AU - Banat, Fawzi
AU - Ponpandian, N.
N1 - Funding Information:
This publication is based upon work supported by the Khalifa University of Science and Technology, United Arab Emirates , under Award No. CIRA-2018-27 .
Funding Information:
This publication is based upon work supported by the Khalifa University of Science and Technology, United Arab Emirates, under Award No. CIRA-2018-27. The authors declare no competing financial interests. The authors declare that the data is available, and can be provided at any stage. The authors state that they have no conflict of interest.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2020/1/31
Y1 - 2020/1/31
N2 - A novel magnetic sorbent, one-dimensional (1D) magnetite hydroxyapatite nanorods on two-dimensional (2D) reduced graphene oxide (MHAp/RGO) was developed for selective separation of hemoglobin (Hb). The MHAp/RGO nanocomposite was prepared through a hydrothermal method at 180 °C for 12 h. Nucleation growth mechanism was analyzed for the formation of the nanocomposite. Electron microscopy analysis showed that the 1D MHAp with average diameter and length of 25 and 200 nm respectively were uniformly grown on 2D RGO sheets. Ternary phases of magnetite, HAp, and RGO in the samples were studied using various physicochemical characterization tools. The obtained MHAp/RGO nanocomposite was used as a high-performance magnetic sorbent for selective adsorption and controlled release of Hb. The as-prepared MHAp/RGO nanocomposite exhibited a maximum adsorption capacity of 1012 mg g−1 at near the isoelectric point (pI(Hb)) of Hb (pH 7.0). Hb adsorption behavior on the nanosorbent was analyzed through the adsorption mechanism, equilibrium isotherms, and kinetic models. Also, the adsorbed Hb on the nanosorbent could be effectively recovered up to 86% at pH 4.0 within 45 min. The present study revealed that the developed magnetic sorbent shows a potential scope for pH-dependent Hb protein isolation and release, thus making it suitable for biomedical applications.
AB - A novel magnetic sorbent, one-dimensional (1D) magnetite hydroxyapatite nanorods on two-dimensional (2D) reduced graphene oxide (MHAp/RGO) was developed for selective separation of hemoglobin (Hb). The MHAp/RGO nanocomposite was prepared through a hydrothermal method at 180 °C for 12 h. Nucleation growth mechanism was analyzed for the formation of the nanocomposite. Electron microscopy analysis showed that the 1D MHAp with average diameter and length of 25 and 200 nm respectively were uniformly grown on 2D RGO sheets. Ternary phases of magnetite, HAp, and RGO in the samples were studied using various physicochemical characterization tools. The obtained MHAp/RGO nanocomposite was used as a high-performance magnetic sorbent for selective adsorption and controlled release of Hb. The as-prepared MHAp/RGO nanocomposite exhibited a maximum adsorption capacity of 1012 mg g−1 at near the isoelectric point (pI(Hb)) of Hb (pH 7.0). Hb adsorption behavior on the nanosorbent was analyzed through the adsorption mechanism, equilibrium isotherms, and kinetic models. Also, the adsorbed Hb on the nanosorbent could be effectively recovered up to 86% at pH 4.0 within 45 min. The present study revealed that the developed magnetic sorbent shows a potential scope for pH-dependent Hb protein isolation and release, thus making it suitable for biomedical applications.
KW - Biomedical applications
KW - Controlled release
KW - Hemoglobin separation
KW - Magnetic sorbents
KW - Ternary nanocomposites
UR - http://www.scopus.com/inward/record.url?scp=85073699237&partnerID=8YFLogxK
U2 - 10.1016/j.apsusc.2019.144215
DO - 10.1016/j.apsusc.2019.144215
M3 - Article
AN - SCOPUS:85073699237
SN - 0169-4332
VL - 501
JO - Applied Surface Science
JF - Applied Surface Science
M1 - 144215
ER -