Synthesis of a fluorescent xanthenic derivative useful for labeling amine residues

Luis Crovetto, Ramon Rios, Jose M. Alvarez-Pez, Jose M. Paredes, Patricia Lozano-Velez, Carmen Del Valle, Eva M. Talayera

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


The 2,5-dioxopyrrolidin-1-yl-4-(3-hydroxy-6-oxo-6H-xanthen-9-yl)-3- methylbenzoate has been synthesized as an amine-reactive derivative able to yield stable covalently labeled biopolymers. The new derivative has been used to label polyribocytidilic acid (5′), poly(C), amine residues. TG-II-poly(C) exhibits monoexponential decay at the physiological pH range. In addition, both steady-state fluorescence intensity and fluorescence decay are also sensitive to solution pH. The large decrease in steady-state fluorescence upon hybridization allows it to be used as a nucleic acid probe in a homogeneous assay format. In summary, we report an efficient synthesis to obtain labeled RNA from commercially available materials in excellent yields.

Original languageBritish English
Pages (from-to)10082-10085
Number of pages4
JournalJournal of Physical Chemistry B
Issue number33
StatePublished - 21 Aug 2008


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