Sustainable cultivation via waste soybean extract for higher vaccenic acid production by purple non-sulfur bacteria

Thị Yến Hoàng, Kuan Shiong Khoo, Hà Lại Thị Ngọc, Quỳnh Trần Thị Thu, Tuyên Thị, Hang Đinh Thị Thu, Ha Chu Hoàng, Sasikala Chinthalapati, Chyi How Lay, Pau Loke Show

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Abstract: The biomass production of Rhodovulum sulfidophilum HPB.6 was optimized via response surface methodology (RSM), and the optimal medium components such as waste soybean extract, yeast extract, and Mg2+ were determined using “one-single-factor-at-one-time” approach. RSM used a three-factor and central composite rotatable design consisting of 21 experimental runs conducted to optimize the final medium components. The optimized conditions were as follows: 2.723 g/L waste soybean extract, 3 g/L yeast extract, and 22 mg/L Mg2+. Under optimized conditions of Rhodovulum sulfidophilum HPB.6, the biomass production was 4.665 ± 0.326 g/L, which was 5.7-folds higher than that under non-optimized conditions. Besides that, the total lipid production was 5.7 times higher corresponding to the increase in biomass productivity. In addition, there was a change in total fatty acid composition with omega 7 and omega 9 which increased from 55.4 to 62.21 and from 3.4 to 9.41, respectively, while omega 6 decreased from 9.79 to 4.54 and omega 3 could not be detected. This exploration of waste soybean under optimized conditions would be a significant impact for the higher biomass production from Rhodovulum sulfidophilum HPB.6. Graphic abstract: [Figure not available: see fulltext.]

Original languageBritish English
Pages (from-to)103-112
Number of pages10
JournalClean Technologies and Environmental Policy
Issue number1
StatePublished - Jan 2021


  • Biomass production
  • Rhodovulum sulfidophilum
  • Vaccenic acid
  • Validated model
  • Waste soybean extract


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