Surfactant/detergent-based liquid biphasic system

Yoong Kit Leong, Veronica Shi Shien Lee, Pau Loke Show

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Scopus citations


The surfactant/detergent-based liquid biphasic system (LBS) is one of the rising technologies for the separation of proteins, cells, viruses, membranes, and other biomolecules. For purification and isolation of biomolecules, liquid-liquid separation using an LBS is an attractive and ideal technology. The LBS is mostly identified as having high water content in both phases; hence it is suitable for partitioning of biomolecules. In this chapter, a brief introduction on surfactants is given, followed by the extraction process for biomolecules using a surfactant-based LBS. Different influences on the extraction of biomolecules, such as the effect of surfactant type, cell-wall properties, and temperature, are discussed. The applications of surfactant-based LBSs are included to prove the feasibility of this extraction process in the real world. The potential of scaling up from lab-scale-based production of the surfactant-based LBS is described in this chapter. This is an important criterion to identify the practicability of large-scale applications of surfactant-based LBS.

Original languageBritish English
Title of host publicationLiquid Biphasic System
Subtitle of host publicationFundamentals and Applications in Bioseparation Technology
Number of pages12
ISBN (Electronic)9780128199862
StatePublished - 1 Jan 2020


  • Detergent
  • Extraction
  • Liquid biphasic system
  • Purification
  • Surfactant


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