Selection, purification, and evaluation of acarbose−an α-glucosidase inhibitor from Actinoplanes sp.

Do Thi Tuyen, Guo Yong Yew, Nguyen Tien Cuong, Le Thanh Hoang, Hoang Thi Yen, Phan Thi Hong Thao, Nguyen Thi Thao, Nguyen Sy le Thanh, Nguyen Thi Hien Trang, Nguyen Thi Trung, Ruqayya Afridi, Dao Thi Mai Anh, Pau Loke Show

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Actinoplanes sp. A1094 strain had been selected for its high production of acarbose from 20 different strains of Actinoplanes sp. can be found in wild. The content for glucosidase inhibitor of acarbose concentration was recorded at 1.12 g/L. The conducted analysis of 16S rRNA sequence of Actinoplanes sp. A1094 showed 99% similar identity to the corresponding sequence of Actinoplanes hulinensis. Acarbose was purified from Actinoplanes hulinensis 1094 with a yield of 8.48%, purity of 98% and further identified by LC/MS and NMR methods (C25H43NO18; m/z: 645.6 g/mol). The purified acarbose was used to evaluate the hypoglycemia in streptozotocin (STZ)-induced diabetic mice model. The purified acarbose reduced postprandial blood glucose level in comparison with Glucobay® as medication for control type 2 diabetes in a combination therapy. Notably, the outcomes of native acarbose on fasting blood glucose levels in mice resemble akin to the commercial product and the acarbose accumulating fermentation and metabolic engineering from the cell gene in which would reduce in production cost. Therefore, acarbose from Actinoplanes hulinensis 1094 could be potentially used to make products for the treatment of type II diabetes.

Original languageBritish English
Article number129167
JournalChemosphere
Volume265
DOIs
StatePublished - Feb 2021

Keywords

  • Acarbose
  • Actinoplanes hulinensis 1094
  • α-glucosidase

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