TY - JOUR
T1 - Requirements for E1A dependent transcription in the yeast Saccharomyces cerevisiae
AU - Yousef, Ahmed F.
AU - Brandl, Christopher J.
AU - Mymryk, Jose S.
N1 - Funding Information:
We thank Drs. Charlie Boone, Joe Martens, Fred Winston, Nikita Avvaku-mov and Jacques Côté for generous gifts of yeast strains. AFY was supported by an Ontario Graduate Scholarship. This work was supported by Canadian Institutes of Health Research grant MOP-74647.
PY - 2009/4/17
Y1 - 2009/4/17
N2 - Background: The human adenovirus type 5 early region 1A (E1A) gene encodes proteins that are potent regulators of transcription. E1A does not bind DNA directly, but is recruited to target promoters by the interaction with sequence specific DNA binding proteins. In mammalian systems, E1A has been shown to contain two regions that can independently induce transcription when fused to a heterologous DNA binding domain. When expressed in Saccharomyces cerevisiae, each of these regions of E1A also acts as a strong transcriptional activator. This allows yeast to be used as a model system to study mechanisms by which E1A stimulates transcription. Results: Using 81 mutant yeast strains, we have evaluated the effect of deleting components of the ADA, COMPASS, CSR, INO80, ISW1, NuA3, NuA4, Mediator, PAF, RSC, SAGA, SAS, SLIK, SWI/SNF and SWR1 transcriptional regulatory complexes on E1A dependent transcription. In addition, we examined the role of histone H2B ubiquitylation by Rad6/Bre1 on transcriptional activation. Conclusion: Our analysis indicates that the two activation domains of E1A function via distinct mechanisms, identify new factors regulating E1A dependent transcription and suggest that yeast can serve as a valid model system for at least some aspects of E1A function.
AB - Background: The human adenovirus type 5 early region 1A (E1A) gene encodes proteins that are potent regulators of transcription. E1A does not bind DNA directly, but is recruited to target promoters by the interaction with sequence specific DNA binding proteins. In mammalian systems, E1A has been shown to contain two regions that can independently induce transcription when fused to a heterologous DNA binding domain. When expressed in Saccharomyces cerevisiae, each of these regions of E1A also acts as a strong transcriptional activator. This allows yeast to be used as a model system to study mechanisms by which E1A stimulates transcription. Results: Using 81 mutant yeast strains, we have evaluated the effect of deleting components of the ADA, COMPASS, CSR, INO80, ISW1, NuA3, NuA4, Mediator, PAF, RSC, SAGA, SAS, SLIK, SWI/SNF and SWR1 transcriptional regulatory complexes on E1A dependent transcription. In addition, we examined the role of histone H2B ubiquitylation by Rad6/Bre1 on transcriptional activation. Conclusion: Our analysis indicates that the two activation domains of E1A function via distinct mechanisms, identify new factors regulating E1A dependent transcription and suggest that yeast can serve as a valid model system for at least some aspects of E1A function.
UR - http://www.scopus.com/inward/record.url?scp=65449137931&partnerID=8YFLogxK
U2 - 10.1186/1471-2199-10-32
DO - 10.1186/1471-2199-10-32
M3 - Article
C2 - 19374760
AN - SCOPUS:65449137931
SN - 1471-2199
VL - 10
JO - BMC Molecular Biology
JF - BMC Molecular Biology
M1 - 32
ER -