Regulation of maternal-fetal heart rates and coupling in mice fetuses

Ahsan H. Khandoker; Chihiro Yoshida; Yoshiyuki Kasahara; Kiyoe Funamoto; Kana Nakanishi; Miyabi Fukase; Keiichi Kanda; Isra Haroun; Kyuichi Niizeki; Yoshitaka Kimura

doi:10.1109/EMBC.2018.8513463

Regulation of maternal-fetal heart rates and coupling in mice fetuses

Ahsan H. Khandoker, Chihiro Yoshida, Yoshiyuki Kasahara, Kiyoe Funamoto, Kana Nakanishi, Miyabi Fukase, Keiichi Kanda, Isra Haroun, Kyuichi Niizeki, Yoshitaka Kimura

Biomedical Engineering & Biotechnology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

8 Scopus citations

Abstract

The aim of this preliminary study is to investigate if there is any evidence of maternal-fetal heart rate coupling in mice fetuses and how the coupling patterns are regulated by vagal nervous system on beat by beat. Total of 6 pregnant female mice were divided into two groups [control (N=3) and vagal blockade (N=3)]. On 17.5-day beat-to-beat heart rates of mothers and fetuses (MHR and FHR) were simultaneously measured for 20 minutes (10 minutes under normal condition and 10 minutes with saline (to control group) and atropine (to the vagal blockade group)) solution by using an invasive maternal and fetal electrocardiogram techniques with needle electrodes. Results show that occasional strong maternal-fetal heart rate coupling (strength was measured by λ appeared and its patterns changed with atropine infusion (no change with saline). Additionally, fisher's exact test shows that changes (increase/decrease from pre to post injection values) in mean, rmssd and power spectral density (PSD) (24 Hz) of MHR, rmssd FHR and PSD (24 Hz) of λ were found to be significantly (p<0.05) associated with treatment types (saline/ atropine). The presented results and protocol allow for the first time in the assessment of autonomic regulation of maternal and fetal heart and their interactions, which will further enhance the value of the mouse as a murine model of heritable human pregnancy and perinatal complications due to maternal conditions.

Original language	British English
Title of host publication	40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	5257-5260
Number of pages	4
ISBN (Electronic)	9781538636466
DOIs	https://doi.org/10.1109/EMBC.2018.8513463
State	Published - 26 Oct 2018
Event	40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018 - Honolulu, United States Duration: 18 Jul 2018 → 21 Jul 2018

Publication series

Name	Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Volume	2018-July
ISSN (Print)	1557-170X

Conference

Conference	40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018
Country/Territory	United States
City	Honolulu
Period	18/07/18 → 21/07/18

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1109/EMBC.2018.8513463

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Khandoker, A. H., Yoshida, C., Kasahara, Y., Funamoto, K., Nakanishi, K., Fukase, M., Kanda, K., Haroun, I., Niizeki, K., & Kimura, Y. (2018). Regulation of maternal-fetal heart rates and coupling in mice fetuses. In 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018 (pp. 5257-5260). Article 8513463 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS; Vol. 2018-July). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/EMBC.2018.8513463

Khandoker, Ahsan H. ; Yoshida, Chihiro ; Kasahara, Yoshiyuki et al. / Regulation of maternal-fetal heart rates and coupling in mice fetuses. 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018. Institute of Electrical and Electronics Engineers Inc., 2018. pp. 5257-5260 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).

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title = "Regulation of maternal-fetal heart rates and coupling in mice fetuses",

abstract = "The aim of this preliminary study is to investigate if there is any evidence of maternal-fetal heart rate coupling in mice fetuses and how the coupling patterns are regulated by vagal nervous system on beat by beat. Total of 6 pregnant female mice were divided into two groups [control (N=3) and vagal blockade (N=3)]. On 17.5-day beat-to-beat heart rates of mothers and fetuses (MHR and FHR) were simultaneously measured for 20 minutes (10 minutes under normal condition and 10 minutes with saline (to control group) and atropine (to the vagal blockade group)) solution by using an invasive maternal and fetal electrocardiogram techniques with needle electrodes. Results show that occasional strong maternal-fetal heart rate coupling (strength was measured by λ appeared and its patterns changed with atropine infusion (no change with saline). Additionally, fisher's exact test shows that changes (increase/decrease from pre to post injection values) in mean, rmssd and power spectral density (PSD) (24 Hz) of MHR, rmssd FHR and PSD (24 Hz) of λ were found to be significantly (p<0.05) associated with treatment types (saline/ atropine). The presented results and protocol allow for the first time in the assessment of autonomic regulation of maternal and fetal heart and their interactions, which will further enhance the value of the mouse as a murine model of heritable human pregnancy and perinatal complications due to maternal conditions.",

author = "Khandoker, {Ahsan H.} and Chihiro Yoshida and Yoshiyuki Kasahara and Kiyoe Funamoto and Kana Nakanishi and Miyabi Fukase and Keiichi Kanda and Isra Haroun and Kyuichi Niizeki and Yoshitaka Kimura",

note = "Funding Information: ACKNOWLEDGMENT This study was partially supported by Khalifa University Internal Research Fund Level 2 awarded to AH Khandoker and Developmental Disorder Data Multi-level Integration Unit of Medical Sciences Innovation Hub Program (MIH), RIKEN. The authors would like to thank Prof K. Niizeki of Yamagata University Japan for the m files of psi and lamda calculation. Publisher Copyright: {\textcopyright} 2018 IEEE.; 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018 ; Conference date: 18-07-2018 Through 21-07-2018",

year = "2018",

month = oct,

day = "26",

doi = "10.1109/EMBC.2018.8513463",

language = "British English",

series = "Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

pages = "5257--5260",

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Khandoker, AH, Yoshida, C, Kasahara, Y, Funamoto, K, Nakanishi, K, Fukase, M, Kanda, K, Haroun, I, Niizeki, K & Kimura, Y 2018, Regulation of maternal-fetal heart rates and coupling in mice fetuses. in 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018., 8513463, Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS, vol. 2018-July, Institute of Electrical and Electronics Engineers Inc., pp. 5257-5260, 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018, Honolulu, United States, 18/07/18. https://doi.org/10.1109/EMBC.2018.8513463

Regulation of maternal-fetal heart rates and coupling in mice fetuses. / Khandoker, Ahsan H.; Yoshida, Chihiro; Kasahara, Yoshiyuki et al.
40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018. Institute of Electrical and Electronics Engineers Inc., 2018. p. 5257-5260 8513463 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS; Vol. 2018-July).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Regulation of maternal-fetal heart rates and coupling in mice fetuses

AU - Khandoker, Ahsan H.

AU - Yoshida, Chihiro

AU - Kasahara, Yoshiyuki

AU - Funamoto, Kiyoe

AU - Nakanishi, Kana

AU - Fukase, Miyabi

AU - Kanda, Keiichi

AU - Haroun, Isra

AU - Niizeki, Kyuichi

AU - Kimura, Yoshitaka

N1 - Funding Information: ACKNOWLEDGMENT This study was partially supported by Khalifa University Internal Research Fund Level 2 awarded to AH Khandoker and Developmental Disorder Data Multi-level Integration Unit of Medical Sciences Innovation Hub Program (MIH), RIKEN. The authors would like to thank Prof K. Niizeki of Yamagata University Japan for the m files of psi and lamda calculation. Publisher Copyright: © 2018 IEEE.

PY - 2018/10/26

Y1 - 2018/10/26

N2 - The aim of this preliminary study is to investigate if there is any evidence of maternal-fetal heart rate coupling in mice fetuses and how the coupling patterns are regulated by vagal nervous system on beat by beat. Total of 6 pregnant female mice were divided into two groups [control (N=3) and vagal blockade (N=3)]. On 17.5-day beat-to-beat heart rates of mothers and fetuses (MHR and FHR) were simultaneously measured for 20 minutes (10 minutes under normal condition and 10 minutes with saline (to control group) and atropine (to the vagal blockade group)) solution by using an invasive maternal and fetal electrocardiogram techniques with needle electrodes. Results show that occasional strong maternal-fetal heart rate coupling (strength was measured by λ appeared and its patterns changed with atropine infusion (no change with saline). Additionally, fisher's exact test shows that changes (increase/decrease from pre to post injection values) in mean, rmssd and power spectral density (PSD) (24 Hz) of MHR, rmssd FHR and PSD (24 Hz) of λ were found to be significantly (p<0.05) associated with treatment types (saline/ atropine). The presented results and protocol allow for the first time in the assessment of autonomic regulation of maternal and fetal heart and their interactions, which will further enhance the value of the mouse as a murine model of heritable human pregnancy and perinatal complications due to maternal conditions.

AB - The aim of this preliminary study is to investigate if there is any evidence of maternal-fetal heart rate coupling in mice fetuses and how the coupling patterns are regulated by vagal nervous system on beat by beat. Total of 6 pregnant female mice were divided into two groups [control (N=3) and vagal blockade (N=3)]. On 17.5-day beat-to-beat heart rates of mothers and fetuses (MHR and FHR) were simultaneously measured for 20 minutes (10 minutes under normal condition and 10 minutes with saline (to control group) and atropine (to the vagal blockade group)) solution by using an invasive maternal and fetal electrocardiogram techniques with needle electrodes. Results show that occasional strong maternal-fetal heart rate coupling (strength was measured by λ appeared and its patterns changed with atropine infusion (no change with saline). Additionally, fisher's exact test shows that changes (increase/decrease from pre to post injection values) in mean, rmssd and power spectral density (PSD) (24 Hz) of MHR, rmssd FHR and PSD (24 Hz) of λ were found to be significantly (p<0.05) associated with treatment types (saline/ atropine). The presented results and protocol allow for the first time in the assessment of autonomic regulation of maternal and fetal heart and their interactions, which will further enhance the value of the mouse as a murine model of heritable human pregnancy and perinatal complications due to maternal conditions.

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Khandoker AH, Yoshida C, Kasahara Y, Funamoto K, Nakanishi K, Fukase M et al. Regulation of maternal-fetal heart rates and coupling in mice fetuses. In 40th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2018. Institute of Electrical and Electronics Engineers Inc. 2018. p. 5257-5260. 8513463. (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). doi: 10.1109/EMBC.2018.8513463

Regulation of maternal-fetal heart rates and coupling in mice fetuses

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