Redox proteomics in the mussel, Mytilus edulis

B. McDonagh; R. Tyther; D. Sheehan

doi:10.1016/j.marenvres.2006.04.001

Redox proteomics in the mussel, Mytilus edulis

B. McDonagh
, R. Tyther
, D. Sheehan

Chemistry

Environmental Research Institute

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Pollutants (e.g. PAHs, metals) cause oxidative stress (OS) by forming reactive oxygen species. Redox proteomics provides a means for identifying protein-specific OS effects in Mytilus edulis. Groups of mussels were sampled from a clean site in Cork Harbour, Ireland and exposed to 1 mM H₂O₂ in holding tanks. Protein extracts of gill and digestive gland were separated by two dimensional electrophoresis and similar protein expression profiles were found. Effects of OS on disulphide bridge patterns were investigated in diagonal gels by separating proteins in non-reducing conditions followed by a second reducing dimension. Immunoprecipitation selected carbonylated and glutathionylated proteins. These methodologies can contribute to redox proteomic studies of pollutant responses in marine organisms.

Original language	British English
Pages (from-to)	S101-S104
Journal	Marine Environmental Research
Volume	62
Issue number	SUPPL. 1
DOIs	https://doi.org/10.1016/j.marenvres.2006.04.001
State	Published - 2006

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

Keywords

Actin
Carbonylation
Glutathione
Mytilus edulis
Oxidative stress
Proteomics

Access to Document

10.1016/j.marenvres.2006.04.001

OpenUrl availability

Full text

Cite this

@article{ff26ceddd42b4b61a6ba4952e1ad5f6b,

title = "Redox proteomics in the mussel, Mytilus edulis",

abstract = "Pollutants (e.g. PAHs, metals) cause oxidative stress (OS) by forming reactive oxygen species. Redox proteomics provides a means for identifying protein-specific OS effects in Mytilus edulis. Groups of mussels were sampled from a clean site in Cork Harbour, Ireland and exposed to 1 mM H2O2 in holding tanks. Protein extracts of gill and digestive gland were separated by two dimensional electrophoresis and similar protein expression profiles were found. Effects of OS on disulphide bridge patterns were investigated in diagonal gels by separating proteins in non-reducing conditions followed by a second reducing dimension. Immunoprecipitation selected carbonylated and glutathionylated proteins. These methodologies can contribute to redox proteomic studies of pollutant responses in marine organisms.",

keywords = "Actin, Carbonylation, Glutathione, Mytilus edulis, Oxidative stress, Proteomics",

author = "B. McDonagh and R. Tyther and D. Sheehan",

year = "2006",

doi = "10.1016/j.marenvres.2006.04.001",

language = "British English",

volume = "62",

pages = "S101--S104",

journal = "Marine Environmental Research",

issn = "0141-1136",

publisher = "Elsevier Ltd",

number = "SUPPL. 1",

}

TY - JOUR

T1 - Redox proteomics in the mussel, Mytilus edulis

AU - McDonagh, B.

AU - Tyther, R.

AU - Sheehan, D.

PY - 2006

Y1 - 2006

N2 - Pollutants (e.g. PAHs, metals) cause oxidative stress (OS) by forming reactive oxygen species. Redox proteomics provides a means for identifying protein-specific OS effects in Mytilus edulis. Groups of mussels were sampled from a clean site in Cork Harbour, Ireland and exposed to 1 mM H2O2 in holding tanks. Protein extracts of gill and digestive gland were separated by two dimensional electrophoresis and similar protein expression profiles were found. Effects of OS on disulphide bridge patterns were investigated in diagonal gels by separating proteins in non-reducing conditions followed by a second reducing dimension. Immunoprecipitation selected carbonylated and glutathionylated proteins. These methodologies can contribute to redox proteomic studies of pollutant responses in marine organisms.

AB - Pollutants (e.g. PAHs, metals) cause oxidative stress (OS) by forming reactive oxygen species. Redox proteomics provides a means for identifying protein-specific OS effects in Mytilus edulis. Groups of mussels were sampled from a clean site in Cork Harbour, Ireland and exposed to 1 mM H2O2 in holding tanks. Protein extracts of gill and digestive gland were separated by two dimensional electrophoresis and similar protein expression profiles were found. Effects of OS on disulphide bridge patterns were investigated in diagonal gels by separating proteins in non-reducing conditions followed by a second reducing dimension. Immunoprecipitation selected carbonylated and glutathionylated proteins. These methodologies can contribute to redox proteomic studies of pollutant responses in marine organisms.

KW - Actin

KW - Carbonylation

KW - Glutathione

KW - Mytilus edulis

KW - Oxidative stress

KW - Proteomics

UR - https://www.scopus.com/pages/publications/33745111195

U2 - 10.1016/j.marenvres.2006.04.001

DO - 10.1016/j.marenvres.2006.04.001

M3 - Article

C2 - 16684561

AN - SCOPUS:33745111195

SN - 0141-1136

VL - 62

SP - S101-S104

JO - Marine Environmental Research

JF - Marine Environmental Research

IS - SUPPL. 1

ER -

Redox proteomics in the mussel, Mytilus edulis

Abstract

UN SDGs

Keywords

Access to Document

OpenUrl availability

Other files and links

Fingerprint

Cite this