Redox proteomics in the mussel, Mytilus edulis

B. McDonagh; R. Tyther; D. Sheehan

doi:10.1016/j.marenvres.2006.04.001

Redox proteomics in the mussel, Mytilus edulis

B. McDonagh, R. Tyther, D. Sheehan

Chemistry

Environmental Research Institute

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Pollutants (e.g. PAHs, metals) cause oxidative stress (OS) by forming reactive oxygen species. Redox proteomics provides a means for identifying protein-specific OS effects in Mytilus edulis. Groups of mussels were sampled from a clean site in Cork Harbour, Ireland and exposed to 1 mM H₂O₂ in holding tanks. Protein extracts of gill and digestive gland were separated by two dimensional electrophoresis and similar protein expression profiles were found. Effects of OS on disulphide bridge patterns were investigated in diagonal gels by separating proteins in non-reducing conditions followed by a second reducing dimension. Immunoprecipitation selected carbonylated and glutathionylated proteins. These methodologies can contribute to redox proteomic studies of pollutant responses in marine organisms.

Original language	British English
Pages (from-to)	S101-S104
Journal	Marine Environmental Research
Volume	62
Issue number	SUPPL. 1
DOIs	https://doi.org/10.1016/j.marenvres.2006.04.001
State	Published - 2006

Keywords

Actin
Carbonylation
Glutathione
Mytilus edulis
Oxidative stress
Proteomics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.marenvres.2006.04.001

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abstract = "Pollutants (e.g. PAHs, metals) cause oxidative stress (OS) by forming reactive oxygen species. Redox proteomics provides a means for identifying protein-specific OS effects in Mytilus edulis. Groups of mussels were sampled from a clean site in Cork Harbour, Ireland and exposed to 1 mM H2O2 in holding tanks. Protein extracts of gill and digestive gland were separated by two dimensional electrophoresis and similar protein expression profiles were found. Effects of OS on disulphide bridge patterns were investigated in diagonal gels by separating proteins in non-reducing conditions followed by a second reducing dimension. Immunoprecipitation selected carbonylated and glutathionylated proteins. These methodologies can contribute to redox proteomic studies of pollutant responses in marine organisms.",

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AU - McDonagh, B.

AU - Tyther, R.

AU - Sheehan, D.

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AB - Pollutants (e.g. PAHs, metals) cause oxidative stress (OS) by forming reactive oxygen species. Redox proteomics provides a means for identifying protein-specific OS effects in Mytilus edulis. Groups of mussels were sampled from a clean site in Cork Harbour, Ireland and exposed to 1 mM H2O2 in holding tanks. Protein extracts of gill and digestive gland were separated by two dimensional electrophoresis and similar protein expression profiles were found. Effects of OS on disulphide bridge patterns were investigated in diagonal gels by separating proteins in non-reducing conditions followed by a second reducing dimension. Immunoprecipitation selected carbonylated and glutathionylated proteins. These methodologies can contribute to redox proteomic studies of pollutant responses in marine organisms.

KW - Actin

KW - Carbonylation

KW - Glutathione

KW - Mytilus edulis

KW - Oxidative stress

KW - Proteomics

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AN - SCOPUS:33745111195

SN - 0141-1136

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Redox proteomics in the mussel, Mytilus edulis

Abstract

Keywords

UN SDGs

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