Redox Modulation of Integin αIIbβ3 Involves a Novel Allosteric Regulation of Its Thiol Isomerase Activity

Geraldine M. Walsh, David Sheehan, Anthony Kinsella, Niamh Moran, Sarah O'Neill

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

The molecular mechanisms involved in regulating the activation-dependent conformational switch in integrins are not known although recent evidence suggests that integrins are a direct target for redox modulation. We have identified an endogenous integrin thiol isomerase activity that may be responsible for regulating integrin activation states. The purpose of this study was to examine the effects of redox conditions elicited by nitric oxide and glutathione on the thiol isomerase activity of the platelet integrin αIIbβ3 and also on the activation status of this integrin in intact platelets. The universal integrin activator, Mn 2+, stimulates the thiol isomerase activity in purified α IIbβ3. Kinetic analysis reveals that α IIbβ3 is an allosteric enzyme which displays positive cooperativity in the presence of Mn2+ with an apparent Hill coefficient of 1.9. Also, addition of Mn2+ to platelets results solely in activation of the integrin as demonstrated by the binding of the antibody PAC-1. The addition of the nitric oxide donors SNP, SIN-1, and SNOAC in combination with glutathione can directly reverse the activation state of the platelet integrin induced by Mn2+. These compounds have no effect on platelet secretory responses indicating a direct effect on the integrin. In the presence of nitric oxide and glutathione, the enzymatic activity of αIIbβ3 also displays positive cooperativity (apparent Hill coefficient of 1.9), and a significant increase in the saturability of the enzyme was observed. Thus, redox agents simultaneously modulate the thiol isomerase activity of purified αIIbβ 3 and its active conformation in intact platelets, suggesting a molecular mechanism for integrin regulation.

Original languageBritish English
Pages (from-to)473-480
Number of pages8
JournalBiochemistry
Volume43
Issue number2
DOIs
StatePublished - 20 Jan 2004

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