Recovery of human interferon alpha-2b from recombinant Escherichia coli using alcohol/salt-based aqueous two-phase systems

Yu Kiat Lin, Chien Wei Ooi, Joo Shun Tan, Pau Loke Show, Arbakariya Ariff, Tau Chuan Ling

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

The purification of intracellular human recombinant interferon-alpha2b (IFN-α2b) from Escherichia coli (E. coli) was studied using alcohol/salt aqueous two-phase system (ATPS). The influences of nine biphasic systems comprising alcohol-based top phase (ethanol, 1-propanol and 2-propanol) and salt-based bottom phase (ammonium sulfate, di-potassium hydrogen phosphate and monosodium citrate) on IFN-α2b purification were studied. The results showed that the optimum condition for purification of IFN-α2b was achieved in ATPS composed of 18% (w/w) 2-propanol with 22% (w/w) ammonium sulfate in the presence of 1% (w/w) sodium chloride (NaCl). The purified IFN-α2b recorded a purification factor (PF) of 16.24 with the yield of 74.64%.

Original languageBritish English
Pages (from-to)362-366
Number of pages5
JournalSeparation and Purification Technology
Volume120
DOIs
StatePublished - 2013

Keywords

  • Alcohol/salt system
  • Aqueous two-phase system
  • Interferon-alpha2b
  • Purification

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