Proteomic identification of tyrosine nitration targets in kidney of spontaneously hypertensive rats

Raymond Tyther, Ahmad Ahmeda, Edward Johns, David Sheehan

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Nitrosative and oxidative stress are implicated in the development of hypertension. Events in the renal medulla may play a key role in the development and progression of hypertension. This may arise through disruption of nitric oxide signalling in the medulla and be accompanied by enhanced nitrosative and oxidative stress as indicated by the presence of proteins containing 3-nitrotyrosine. Here we demonstrate enhanced protein nitration in the medulla of spontaneously hypertensive rats. We have identified several nitrated proteins with both varied subcellular location and functional roles. These proteins are involved in nitric oxide signalling, antioxidant defense and energy metabolism. Moreover, increased nitration was observed in conjunction with enhanced oxidative damage as evidenced by the presence of protein carbonyl oxidative stress biomarkers. Our results suggest that kidney medulla is subject to enhanced nitrosative and oxidative stress, and that resulting protein modifications may contribute to the progression of hypertension.

Original languageBritish English
Pages (from-to)4555-4564
Number of pages10
JournalProteomics
Volume7
Issue number24
DOIs
StatePublished - Dec 2007

Keywords

  • 3-Nitrotyrosine
  • Carbonylation
  • Hypertension
  • Kidney
  • Rat

Fingerprint

Dive into the research topics of 'Proteomic identification of tyrosine nitration targets in kidney of spontaneously hypertensive rats'. Together they form a unique fingerprint.

Cite this