Proteome analysis of diseased joints from mice suffering from collagen-induced arthritis

Peter Lorenz, Marcus Bantscheff, Saleh M. Ibrahim, Hans Jürgen Thiesen, Michael O. Glocker

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Strains of mice that are susceptible to autoimmunity have provided experimental models to analyze the molecular basis for the complex multifactorial inheritance of human autoimmune disease. In this study proteins associated with collagen-induced arthritis (CIA) in mice were experimentally identified using a global proteomics approach. Two-dimensional gels of proteins from inflamed and non-inflamed joints showed a distinguished protein profile visualizing about 530 Coomassie-stained protein spots in the pH 4-7 range. A total of 76 spots were identified by peptide mass fingerprinting with good confidence. They included proteins of cytoskeletal origin, chaperones, enzymes and also some signal transduction molecules. Comparison to gels from non-inflamed paws pointed to proteins that were differentially expressed between the control and diseased state. Ferritin light chain and antioxidant protein 2 were slightly more abundant, lymphoid enhancer binding factor 1 slightly, but significantly, less abundant in inflamed paws. Fourteen of the identified proteins were the products of genes that had increased transcript levels in the diseased state. However, on the protein level no significant differences were found in comparison to the controls. This study provides us with the framework for more detailed approaches to understanding the complex disease arthritis that go beyond global proteomics.

Original languageBritish English
Pages (from-to)1622-1632
Number of pages11
JournalClinical Chemistry and Laboratory Medicine
Volume41
Issue number12
DOIs
StatePublished - 2003

Keywords

  • Arthritis
  • MALDI-MS
  • MALDI-PSD-TOF-MS
  • Mass spectrometry: MS
  • Peptide sequencing protein identification
  • Proteomics

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