Proteome analysis of brain in murine experimental autoimmune encephalomyelitis

Abolhassan Shahzadeh Fazeli; Davood Nasrabadi; Mohammad Hossein Sanati; Alireza Pouya; Saleh M. Ibrahim; Hossein Baharvand; Ghasem Hosseini Salekdeh

doi:10.1002/pmic.200900507

Proteome analysis of brain in murine experimental autoimmune encephalomyelitis

Abolhassan Shahzadeh Fazeli, Davood Nasrabadi, Mohammad Hossein Sanati, Alireza Pouya, Saleh M. Ibrahim, Hossein Baharvand, Ghasem Hosseini Salekdeh

College of Medicine and Health Sciences

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Multiple sclerosis is considered a prototype inflammatory autoimmune disorder of the CNS. Experimental autoimmune encephalomyelitis (EAE) induced by myelin oligodendrocyte glycoprotein is one of the best-characterized animal models of multiple sclerosis. Comprehensive understanding of gene expression in EAE can help identify genes that are important in drug response and pathogenesis. We applied a 2-DE-based proteomics approach to analyze the protein expression pattern of the brain in healthy and EAE samples. Of more than 1000 protein spots we analyzed, 70 showed reproducible and significant changes in EAE compared to controls. Of these, 42 protein spots could be identified using MALDI TOF-TOF-MS. They included mitochondrial and structural proteins as well as proteins involved in ionic and neurotransmitter release, blood barriers, apoptosis, and signal transduction. The possible role of these proteins in the responses of mice to animal models of multiple sclerosis is discussed.

Original language	British English
Pages (from-to)	2822-2832
Number of pages	11
Journal	Proteomics
Volume	10
Issue number	15
DOIs	https://doi.org/10.1002/pmic.200900507
State	Published - Aug 2010

Keywords

2-DE
Cell biology
Expression profiling
Multiple sclerosis

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10.1002/pmic.200900507

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title = "Proteome analysis of brain in murine experimental autoimmune encephalomyelitis",

abstract = "Multiple sclerosis is considered a prototype inflammatory autoimmune disorder of the CNS. Experimental autoimmune encephalomyelitis (EAE) induced by myelin oligodendrocyte glycoprotein is one of the best-characterized animal models of multiple sclerosis. Comprehensive understanding of gene expression in EAE can help identify genes that are important in drug response and pathogenesis. We applied a 2-DE-based proteomics approach to analyze the protein expression pattern of the brain in healthy and EAE samples. Of more than 1000 protein spots we analyzed, 70 showed reproducible and significant changes in EAE compared to controls. Of these, 42 protein spots could be identified using MALDI TOF-TOF-MS. They included mitochondrial and structural proteins as well as proteins involved in ionic and neurotransmitter release, blood barriers, apoptosis, and signal transduction. The possible role of these proteins in the responses of mice to animal models of multiple sclerosis is discussed.",

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AU - Fazeli, Abolhassan Shahzadeh

AU - Nasrabadi, Davood

AU - Sanati, Mohammad Hossein

AU - Pouya, Alireza

AU - Ibrahim, Saleh M.

AU - Baharvand, Hossein

AU - Salekdeh, Ghasem Hosseini

PY - 2010/8

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AB - Multiple sclerosis is considered a prototype inflammatory autoimmune disorder of the CNS. Experimental autoimmune encephalomyelitis (EAE) induced by myelin oligodendrocyte glycoprotein is one of the best-characterized animal models of multiple sclerosis. Comprehensive understanding of gene expression in EAE can help identify genes that are important in drug response and pathogenesis. We applied a 2-DE-based proteomics approach to analyze the protein expression pattern of the brain in healthy and EAE samples. Of more than 1000 protein spots we analyzed, 70 showed reproducible and significant changes in EAE compared to controls. Of these, 42 protein spots could be identified using MALDI TOF-TOF-MS. They included mitochondrial and structural proteins as well as proteins involved in ionic and neurotransmitter release, blood barriers, apoptosis, and signal transduction. The possible role of these proteins in the responses of mice to animal models of multiple sclerosis is discussed.

KW - 2-DE

KW - Cell biology

KW - Expression profiling

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Proteome analysis of brain in murine experimental autoimmune encephalomyelitis

Abstract

Keywords

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