Proteome analysis of brain in murine experimental autoimmune encephalomyelitis

Abolhassan Shahzadeh Fazeli, Davood Nasrabadi, Mohammad Hossein Sanati, Alireza Pouya, Saleh M. Ibrahim, Hossein Baharvand, Ghasem Hosseini Salekdeh

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Multiple sclerosis is considered a prototype inflammatory autoimmune disorder of the CNS. Experimental autoimmune encephalomyelitis (EAE) induced by myelin oligodendrocyte glycoprotein is one of the best-characterized animal models of multiple sclerosis. Comprehensive understanding of gene expression in EAE can help identify genes that are important in drug response and pathogenesis. We applied a 2-DE-based proteomics approach to analyze the protein expression pattern of the brain in healthy and EAE samples. Of more than 1000 protein spots we analyzed, 70 showed reproducible and significant changes in EAE compared to controls. Of these, 42 protein spots could be identified using MALDI TOF-TOF-MS. They included mitochondrial and structural proteins as well as proteins involved in ionic and neurotransmitter release, blood barriers, apoptosis, and signal transduction. The possible role of these proteins in the responses of mice to animal models of multiple sclerosis is discussed.

Original languageBritish English
Pages (from-to)2822-2832
Number of pages11
JournalProteomics
Volume10
Issue number15
DOIs
StatePublished - Aug 2010

Keywords

  • 2-DE
  • Cell biology
  • Expression profiling
  • Multiple sclerosis

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