TY - JOUR
T1 - Protein Adsorption and Coordination-Based End-Tethering of Functional Polymers on Metal-Phenolic Network Films
AU - Tardy, Blaise L.
AU - Richardson, Joseph J.
AU - Nithipipat, Vichida
AU - Kempe, Kristian
AU - Guo, Junling
AU - Cho, Kwun Lun
AU - Rahim, Md Arifur
AU - Ejima, Hirotaka
AU - Caruso, Frank
N1 - Funding Information:
*E-mail: [email protected]. ORCID Blaise L. Tardy: 0000-0002-7648-0376 Kristian Kempe: 0000-0002-0136-9403 Junling Guo: 0000-0002-2948-880X Hirotaka Ejima: 0000-0002-4965-9493 Frank Caruso: 0000-0002-0197-497X Present Addresses ⊥Department of Biomass Chemistry and Engineering, Sichuan University, Chengdu 610065, China. †Department of Bioproducts and Biosystems, School of Chemical Engineering, Aalto University, FI-00076 Aalto, Finland. ‡ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Victoria 3052, Australia. §Department of Materials Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8656, Japan. Author Contributions The manuscript was written through contributions of all authors. All authors have given approval to the final version of the manuscript. Funding This research was conducted and funded by the Australian Research Council (ARC) Centre of Excellence in Convergent Bio-Nano Science and Technology (Project No. CE140100036) and an ARC Discovery Project (DP170103331). F.C. acknowledges the award of a National Health and Medical Research Council Senior Principal Research Fellowship (APP1135806). K.K. acknowledges the Alexander von Humboldt Foundation for a Feodor Lynen Research Fellowship. This work was performed in part at the Materials Characterisation and Fabrication Platform at The University of Melbourne and the Victorian Node of the Australian National Fabrication Facility. Notes The authors declare no competing financial interest.
Publisher Copyright:
© 2019 American Chemical Society.
PY - 2019/3/11
Y1 - 2019/3/11
N2 - Metal-phenolic network (MPN) coatings have generated increasing interest owing to their biologically inspired nature, facile fabrication, and near-universal adherence, especially for biomedical applications. However, a key issue in biomedicine is protein fouling, and the adsorption of proteins on tannic acid-based MPNs remains to be comprehensively studied. Herein, we investigate the interaction of specific biomedically relevant proteins in solution (e.g., bovine serum albumin (BSA), immunoglobulin G (IgG), fibrinogen) and complex biological media (serum) using layer-by-layer-assembled tannic acid/FeIII MPN films. When FeIII was the outermost layer, galloyl-modified poly(2-ethyl-2-oxazoline) (P(EtOx)-Gal) could be grafted to the films through coordination bonds. Protein fouling and bacterial adhesion were greatly suppressed after functionalization with P(EtOx)-Gal and the mass of adsorbed protein was reduced by 79%. Interestingly, larger proteins adsorbed more on both the MPNs and P(EtOx)-functionalized MPNs. This study provides fundamental information on the interactions of MPNs with single proteins, mixtures of proteins as encountered in serum, and the noncovalent, coordination-based, functionalization of MPN films.
AB - Metal-phenolic network (MPN) coatings have generated increasing interest owing to their biologically inspired nature, facile fabrication, and near-universal adherence, especially for biomedical applications. However, a key issue in biomedicine is protein fouling, and the adsorption of proteins on tannic acid-based MPNs remains to be comprehensively studied. Herein, we investigate the interaction of specific biomedically relevant proteins in solution (e.g., bovine serum albumin (BSA), immunoglobulin G (IgG), fibrinogen) and complex biological media (serum) using layer-by-layer-assembled tannic acid/FeIII MPN films. When FeIII was the outermost layer, galloyl-modified poly(2-ethyl-2-oxazoline) (P(EtOx)-Gal) could be grafted to the films through coordination bonds. Protein fouling and bacterial adhesion were greatly suppressed after functionalization with P(EtOx)-Gal and the mass of adsorbed protein was reduced by 79%. Interestingly, larger proteins adsorbed more on both the MPNs and P(EtOx)-functionalized MPNs. This study provides fundamental information on the interactions of MPNs with single proteins, mixtures of proteins as encountered in serum, and the noncovalent, coordination-based, functionalization of MPN films.
UR - http://www.scopus.com/inward/record.url?scp=85062690872&partnerID=8YFLogxK
U2 - 10.1021/acs.biomac.9b00006
DO - 10.1021/acs.biomac.9b00006
M3 - Article
C2 - 30794387
AN - SCOPUS:85062690872
SN - 1525-7797
VL - 20
SP - 1421
EP - 1428
JO - Biomacromolecules
JF - Biomacromolecules
IS - 3
ER -