Production of bio-hydrogen from dairy wastewater using pretreated landfill leachate sludge as an inoculum

Yee Meng Wong, Pau Loke Show, Ta Yeong Wu, Hui Yi Leong, Shaliza Ibrahim, Joon Ching Juan

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Bio-hydrogen production from wastewater using sludge as inoculum is a sustainable approach for energy production. This study investigated the influence of initial pH and temperature on bio-hydrogen production from dairy wastewater using pretreated landfill leachate sludge (LLS) as an inoculum. The maximum yield of 113.2 ± 2.9 mmol H 2 /g chemical oxygen demand (COD) (12.8 ± 0.3 mmol H 2 /g carbohydrates) was obtained at initial pH 6 and 37 °C. The main products of volatile fatty acids were acetate and butyrate with the ratio of acetate:butyrate was 0.4. At optimum condition, Gibb's free energy was estimated at −40 kJ/mol, whereas the activation enthalpy and entropy were 65 kJ/mol and 0.128 kJ/mol/l, respectively. These thermodynamic quantities suggest that bio-hydrogen production from dairy wastewater using pretreated LLS as inoculum was effective and efficient. In addition, genomic and bioinformatics analyses were performed in this study.

Original languageBritish English
Pages (from-to)150-159
Number of pages10
JournalJournal of Bioscience and Bioengineering
Volume127
Issue number2
DOIs
StatePublished - Feb 2019

Keywords

  • Arrhenius
  • Bio-hydrogen
  • Enthalpy
  • Entropy
  • Gibb's free energy
  • Modified gompertz model

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