Primary capture of Bacillus subtilis xylanase from crude feedstock using alcohol/salt liquid biphasic flotation

Phei Er Kee, Lie San Cheah, Pik Ki Wan, Pau Loke Show, John Chi Wei Lan, Yin Hui Chow, Hui Suan Ng

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Liquid biphasic flotation (LBF) offers advanced downstream processing of bioproducts by floating of gas bubbles within the biphasic system to enhance the separation efficiency of target bioproducts. In this study, the feasibility of alcohol/salt LBF for primary capture of extracellular Bacillus subtilis xylanase from crude feedstock was investigated. The effects of types of phase-forming components; phase composition; concentration of crude feedstock load; and flotation time on the recovery performance of xylanase in LBF were evaluated. The recovery of xylanase was achieved in LBF comprised of 28 % (w/w) 2-propanol, 22 % (w/w) ammonium sulfate and 10 % (w/w) B. subtilis crude feedstock load. A maximum partition coefficient (Ke) of 13.29 ± 0.14, selectivity (S) of 24.71 ± 0.96 and recovery yield (YT) of 90.03 % ± 0.10 were recorded in the optimum 2-propanol/sulfate LBF with flotation time of 20 min. SDS-PAGE analysis revealed that B. subtilis xylanase was successfully recovered in the 2-propanol-rich top phase with high purity. The findings demonstrated that LBF is a practicable technology for the large-scale production of microbial xylanase in a one-step operation.

Original languageBritish English
Article number107835
JournalBiochemical Engineering Journal
Volume165
DOIs
StatePublished - 15 Jan 2021

Keywords

  • Alcohol
  • Bacillus subtilis
  • Liquid biphasic flotation
  • Recovery
  • Xylanase

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