Preparation of chitosan grafted graphite composite for sensitive detection of dopamine in biological samples

Selvakumar Palanisamy; Kokulnathan Thangavelu; Shen Ming Chen; P. Gnanaprakasam; Vijayalakshmi Velusamy; Xiao Heng Liu

doi:10.1016/j.carbpol.2016.05.076

Preparation of chitosan grafted graphite composite for sensitive detection of dopamine in biological samples

Selvakumar Palanisamy, Kokulnathan Thangavelu, Shen Ming Chen, P. Gnanaprakasam, Vijayalakshmi Velusamy, Xiao Heng Liu

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics. In this work, we describe the preparation of chitosan (CS) biopolymer grafted graphite (GR) composite for the sensitive and lower potential detection of DA in its sub micromolar levels. The composite modified electrode has been used for the detection of DA in biological samples such as human serum and urine. The GR-CS composite modified electrode shows an enhanced oxidation peak current response and low oxidation potential for the detection of DA than that of electrodes modified with bare, GR and CS discretely. Under optimum conditions, the fabricated GR-CS composite modified electrode shows the DPV response of DA in the linear response ranging from 0.03 to 20.06 μM. The detection limit and sensitivity of the sensor were estimated as 0.0045 μM and 6.06 μA μM^-1 cm^-2, respectively.

Original language	British English
Pages (from-to)	401-407
Number of pages	7
Journal	Carbohydrate Polymers
Volume	151
DOIs	https://doi.org/10.1016/j.carbpol.2016.05.076
State	Published - 20 Oct 2016

Keywords

Biopolymer
Chitosan
Differential pulse voltammetry
Dopamine
Electro-oxidation
Graphite

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10.1016/j.carbpol.2016.05.076

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title = "Preparation of chitosan grafted graphite composite for sensitive detection of dopamine in biological samples",

abstract = "The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics. In this work, we describe the preparation of chitosan (CS) biopolymer grafted graphite (GR) composite for the sensitive and lower potential detection of DA in its sub micromolar levels. The composite modified electrode has been used for the detection of DA in biological samples such as human serum and urine. The GR-CS composite modified electrode shows an enhanced oxidation peak current response and low oxidation potential for the detection of DA than that of electrodes modified with bare, GR and CS discretely. Under optimum conditions, the fabricated GR-CS composite modified electrode shows the DPV response of DA in the linear response ranging from 0.03 to 20.06 μM. The detection limit and sensitivity of the sensor were estimated as 0.0045 μM and 6.06 μA μM-1 cm-2, respectively.",

keywords = "Biopolymer, Chitosan, Differential pulse voltammetry, Dopamine, Electro-oxidation, Graphite",

author = "Selvakumar Palanisamy and Kokulnathan Thangavelu and Chen, {Shen Ming} and P. Gnanaprakasam and Vijayalakshmi Velusamy and Liu, {Xiao Heng}",

note = "Funding Information: This project was supported by the Ministry of Science and Technology, Taiwan (Republic of China). Publisher Copyright: {\textcopyright} 2016 Elsevier Ltd. All rights reserved.",

year = "2016",

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doi = "10.1016/j.carbpol.2016.05.076",

language = "British English",

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AU - Palanisamy, Selvakumar

AU - Thangavelu, Kokulnathan

AU - Chen, Shen Ming

AU - Gnanaprakasam, P.

AU - Velusamy, Vijayalakshmi

AU - Liu, Xiao Heng

PY - 2016/10/20

Y1 - 2016/10/20

N2 - The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics. In this work, we describe the preparation of chitosan (CS) biopolymer grafted graphite (GR) composite for the sensitive and lower potential detection of DA in its sub micromolar levels. The composite modified electrode has been used for the detection of DA in biological samples such as human serum and urine. The GR-CS composite modified electrode shows an enhanced oxidation peak current response and low oxidation potential for the detection of DA than that of electrodes modified with bare, GR and CS discretely. Under optimum conditions, the fabricated GR-CS composite modified electrode shows the DPV response of DA in the linear response ranging from 0.03 to 20.06 μM. The detection limit and sensitivity of the sensor were estimated as 0.0045 μM and 6.06 μA μM-1 cm-2, respectively.

AB - The accurate detection of dopamine (DA) levels in biological samples such as human serum and urine are essential indicators in medical diagnostics. In this work, we describe the preparation of chitosan (CS) biopolymer grafted graphite (GR) composite for the sensitive and lower potential detection of DA in its sub micromolar levels. The composite modified electrode has been used for the detection of DA in biological samples such as human serum and urine. The GR-CS composite modified electrode shows an enhanced oxidation peak current response and low oxidation potential for the detection of DA than that of electrodes modified with bare, GR and CS discretely. Under optimum conditions, the fabricated GR-CS composite modified electrode shows the DPV response of DA in the linear response ranging from 0.03 to 20.06 μM. The detection limit and sensitivity of the sensor were estimated as 0.0045 μM and 6.06 μA μM-1 cm-2, respectively.

KW - Biopolymer

KW - Chitosan

KW - Differential pulse voltammetry

KW - Dopamine

KW - Electro-oxidation

KW - Graphite

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SN - 0144-8617

VL - 151

SP - 401

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JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

ER -

Preparation of chitosan grafted graphite composite for sensitive detection of dopamine in biological samples

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Keywords

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