Polymorphism in murine mtATP8 gene correlates with decreased reactive oxygen species in aging hematopoietic cells

Catrin Roolf; Christin Kretzschmar; Katrin Timmer; Anett Sekora; Gudrun Knübel; Hugo Murua Escobar; Georg Fuellen; Saleh M. Ibrahim; Markus Tiedge; Simone Baltrusch; Sarah Müller; Rüdiger Köhling; Christian Junghanss

doi:10.21873/invivo.10991

Polymorphism in murine mtATP8 gene correlates with decreased reactive oxygen species in aging hematopoietic cells

Catrin Roolf, Christin Kretzschmar, Katrin Timmer, Anett Sekora, Gudrun Knübel, Hugo Murua Escobar, Georg Fuellen, Saleh M. Ibrahim, Markus Tiedge, Simone Baltrusch, Sarah Müller, Rüdiger Köhling, Christian Junghanss

College of Medicine and Health Sciences

University Medicine Rostock

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Background: Mitochondrial DNA (mtDNA) encodes for the respiratory chain proteins. Genetic alterations in mtDNA have been described during aging and linked to impaired hematopoiesis. Materials and Methods: We investigated two novel conplastic mouse strains harboring a mitochondrial nt7778 G/T polymorphism leading to an amino acid exchange in respiratory chain complex V. Effects on reactive oxygen species (ROS) and adenosine triphosphate (ATP) levels, as well as bone marrow composition and peripheral blood counts, were investigated during aging (up to 24 month). Results: The polymorphism correlated with significantly decreased ROS levels in aged mice. Effects on hematopoiesis were marginal and not statistically significant: numbers of erythroid cells in bone marrow, as well as mean corpuscular hemoglobin, tended to decrease over time. Conclusion: The investigated polymorphism is associated with decreased ROS levels in aged hematopoietic cells but does not significantly influence hematopoiesis itself.

Original language	British English
Pages (from-to)	751-760
Number of pages	10
Journal	In Vivo
Volume	30
Issue number	6
DOIs	https://doi.org/10.21873/invivo.10991
State	Published - 1 Nov 2016

Keywords

Conplastic mice
Hematopoiesis
mtDNA
ROS
Stem cells

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.21873/invivo.10991

Cite this

@article{0a9ac0d8ff6e4200bb140eee239c85d4,

title = "Polymorphism in murine mtATP8 gene correlates with decreased reactive oxygen species in aging hematopoietic cells",

abstract = "Background: Mitochondrial DNA (mtDNA) encodes for the respiratory chain proteins. Genetic alterations in mtDNA have been described during aging and linked to impaired hematopoiesis. Materials and Methods: We investigated two novel conplastic mouse strains harboring a mitochondrial nt7778 G/T polymorphism leading to an amino acid exchange in respiratory chain complex V. Effects on reactive oxygen species (ROS) and adenosine triphosphate (ATP) levels, as well as bone marrow composition and peripheral blood counts, were investigated during aging (up to 24 month). Results: The polymorphism correlated with significantly decreased ROS levels in aged mice. Effects on hematopoiesis were marginal and not statistically significant: numbers of erythroid cells in bone marrow, as well as mean corpuscular hemoglobin, tended to decrease over time. Conclusion: The investigated polymorphism is associated with decreased ROS levels in aged hematopoietic cells but does not significantly influence hematopoiesis itself.",

keywords = "Conplastic mice, Hematopoiesis, mtDNA, ROS, Stem cells",

author = "Catrin Roolf and Christin Kretzschmar and Katrin Timmer and Anett Sekora and Gudrun Kn{\"u}bel and Escobar, {Hugo Murua} and Georg Fuellen and Ibrahim, {Saleh M.} and Markus Tiedge and Simone Baltrusch and Sarah M{\"u}ller and R{\"u}diger K{\"o}hling and Christian Junghanss",

year = "2016",

month = nov,

day = "1",

doi = "10.21873/invivo.10991",

language = "British English",

volume = "30",

pages = "751--760",

journal = "In Vivo",

issn = "0258-851X",

publisher = "International Institute of Anticancer Research",

number = "6",

}

TY - JOUR

T1 - Polymorphism in murine mtATP8 gene correlates with decreased reactive oxygen species in aging hematopoietic cells

AU - Roolf, Catrin

AU - Kretzschmar, Christin

AU - Timmer, Katrin

AU - Sekora, Anett

AU - Knübel, Gudrun

AU - Escobar, Hugo Murua

AU - Fuellen, Georg

AU - Ibrahim, Saleh M.

AU - Tiedge, Markus

AU - Baltrusch, Simone

AU - Müller, Sarah

AU - Köhling, Rüdiger

AU - Junghanss, Christian

PY - 2016/11/1

Y1 - 2016/11/1

N2 - Background: Mitochondrial DNA (mtDNA) encodes for the respiratory chain proteins. Genetic alterations in mtDNA have been described during aging and linked to impaired hematopoiesis. Materials and Methods: We investigated two novel conplastic mouse strains harboring a mitochondrial nt7778 G/T polymorphism leading to an amino acid exchange in respiratory chain complex V. Effects on reactive oxygen species (ROS) and adenosine triphosphate (ATP) levels, as well as bone marrow composition and peripheral blood counts, were investigated during aging (up to 24 month). Results: The polymorphism correlated with significantly decreased ROS levels in aged mice. Effects on hematopoiesis were marginal and not statistically significant: numbers of erythroid cells in bone marrow, as well as mean corpuscular hemoglobin, tended to decrease over time. Conclusion: The investigated polymorphism is associated with decreased ROS levels in aged hematopoietic cells but does not significantly influence hematopoiesis itself.

AB - Background: Mitochondrial DNA (mtDNA) encodes for the respiratory chain proteins. Genetic alterations in mtDNA have been described during aging and linked to impaired hematopoiesis. Materials and Methods: We investigated two novel conplastic mouse strains harboring a mitochondrial nt7778 G/T polymorphism leading to an amino acid exchange in respiratory chain complex V. Effects on reactive oxygen species (ROS) and adenosine triphosphate (ATP) levels, as well as bone marrow composition and peripheral blood counts, were investigated during aging (up to 24 month). Results: The polymorphism correlated with significantly decreased ROS levels in aged mice. Effects on hematopoiesis were marginal and not statistically significant: numbers of erythroid cells in bone marrow, as well as mean corpuscular hemoglobin, tended to decrease over time. Conclusion: The investigated polymorphism is associated with decreased ROS levels in aged hematopoietic cells but does not significantly influence hematopoiesis itself.

KW - Conplastic mice

KW - Hematopoiesis

KW - mtDNA

KW - ROS

KW - Stem cells

UR - http://www.scopus.com/inward/record.url?scp=84994205147&partnerID=8YFLogxK

U2 - 10.21873/invivo.10991

DO - 10.21873/invivo.10991

M3 - Article

C2 - 27815458

AN - SCOPUS:84994205147

SN - 0258-851X

VL - 30

SP - 751

EP - 760

JO - In Vivo

JF - In Vivo

IS - 6

ER -

Polymorphism in murine mtATP8 gene correlates with decreased reactive oxygen species in aging hematopoietic cells

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this