Abstract
The polymer-based liquid biphasic system (LBS) is a conventional liquid-liquid extraction, separation, and purification technique for biomolecules that utilizes an organic-aqueous phase system such as polymer-polymer (e.g., polyethylene glycol, dextran) or polymer-salt (e.g., phosphate based, sulfate based). This approach is a promising and useful technique that is widely used for extraction of various biomolecules. The basic principles of the polymer-based LBS for the extraction of various biomolecules are discussed. The various key parameters, such as molecular weight, size and concentration of polymer and salt, pH system, tie-line length, and temperature are comprehensively evaluated. Moreover, the application of polymer-based LBSs for the extraction of various biomolecules from complex crude feedstock and the experimental research utilizing polymer-based LBSs are summarized. This chapter provides a theoretical and fundamental knowledge for researchers who are dealing with separation and purification technology.
| Original language | British English |
|---|---|
| Title of host publication | Liquid Biphasic System |
| Subtitle of host publication | Fundamentals and Applications in Bioseparation Technology |
| Publisher | Elsevier |
| Pages | 17-37 |
| Number of pages | 21 |
| ISBN (Electronic) | 9780128199862 |
| DOIs | |
| State | Published - 1 Jan 2020 |
Keywords
- Biomolecules
- Extraction efficiency
- Liquid biphasic system
- Polymer-based
- Purification