TY - JOUR
T1 - Poly-ε-caprolactone/fibrin-alginate scaffold
T2 - A new pro-angiogenic composite biomaterial for the treatment of bone defects
AU - Ren, Jiongyu
AU - Kohli, Nupur
AU - Sharma, Vaibhav
AU - Shakouri, Taleen
AU - Keskin-Erdogan, Zalike
AU - Saifzadeh, Siamak
AU - Brierly, Gary I.
AU - Knowles, Jonathan C.
AU - Woodruff, Maria A.
AU - García-Gareta, Elena
N1 - Funding Information:
Funding: This research was funded by the Restoration of Appearance and Function Trust (UK, registered charity number 299811) charitable funds and the Australian Research Council Linkage Projects funding scheme (LP130100461).
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/10/1
Y1 - 2021/10/1
N2 - We hypothesized that a composite of 3D porous melt-electrowritten poly-ε-caprolactone (PCL) coated throughout with a porous and slowly biodegradable fibrin/alginate (FA) matrix would accelerate bone repair due to its angiogenic potential. Scanning electron microscopy showed that the open pore structure of the FA matrix was maintained in the PCL/FA composites. Fourier transform infrared spectroscopy and differential scanning calorimetry showed complete coverage of the PCL fibres by FA, and the PCL/FA crystallinity was decreased compared with PCL. In vitro cell work with osteoprogenitor cells showed that they preferentially bound to the FA component and proliferated on all scaffolds over 28 days. A chorioallantoic membrane assay showed more blood vessel infiltration into FA and PCL/FA compared with PCL, and a significantly higher number of bifurcation points for PCL/FA compared with both FA and PCL. Implantation into a rat cranial defect model followed by microcomputed tomography, histology, and immunohistochemistry after 4-and 12-weeks post operation showed fast early bone formation at week 4, with significantly higher bone formation for FA and PCL/FA compared with PCL. However, this phenomenon was not extrapolated to week 12. Therefore, for long-term bone regeneration, tuning of FA degradation to ensure syncing with new bone formation is likely necessary.
AB - We hypothesized that a composite of 3D porous melt-electrowritten poly-ε-caprolactone (PCL) coated throughout with a porous and slowly biodegradable fibrin/alginate (FA) matrix would accelerate bone repair due to its angiogenic potential. Scanning electron microscopy showed that the open pore structure of the FA matrix was maintained in the PCL/FA composites. Fourier transform infrared spectroscopy and differential scanning calorimetry showed complete coverage of the PCL fibres by FA, and the PCL/FA crystallinity was decreased compared with PCL. In vitro cell work with osteoprogenitor cells showed that they preferentially bound to the FA component and proliferated on all scaffolds over 28 days. A chorioallantoic membrane assay showed more blood vessel infiltration into FA and PCL/FA compared with PCL, and a significantly higher number of bifurcation points for PCL/FA compared with both FA and PCL. Implantation into a rat cranial defect model followed by microcomputed tomography, histology, and immunohistochemistry after 4-and 12-weeks post operation showed fast early bone formation at week 4, with significantly higher bone formation for FA and PCL/FA compared with PCL. However, this phenomenon was not extrapolated to week 12. Therefore, for long-term bone regeneration, tuning of FA degradation to ensure syncing with new bone formation is likely necessary.
KW - Alginate
KW - Angiogenesis
KW - Bone regeneration
KW - Fibrin
KW - Polycaprolactone
UR - http://www.scopus.com/inward/record.url?scp=85116394122&partnerID=8YFLogxK
U2 - 10.3390/polym13193399
DO - 10.3390/polym13193399
M3 - Article
AN - SCOPUS:85116394122
SN - 2073-4360
VL - 13
JO - Polymers
JF - Polymers
IS - 19
M1 - 3399
ER -