Pilot-scale aqueous two-phase floatation for direct recovery of lipase derived from Burkholderia cepacia strain ST8

Preshna Mathiazakan; Seah Yieng Shing; Sia Sin Ying; Heng Keng Kek; Malcolm S.Y. Tang; Pau Loke Show; Chien Wei Ooi; Tau Chuan Ling

doi:10.1016/j.seppur.2016.07.017

Pilot-scale aqueous two-phase floatation for direct recovery of lipase derived from Burkholderia cepacia strain ST8

Preshna Mathiazakan, Seah Yieng Shing, Sia Sin Ying, Heng Keng Kek, Malcolm S.Y. Tang, Pau Loke Show, Chien Wei Ooi, Tau Chuan Ling

Chemical and Petroleum Engineering

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

The efficient and economical pilot-scale production of lipase is necessary for meeting the growing demand of lipase especially in bioprocessing industry. Aqueous two-phase flotation (ATPF) is a purification technique that can be applied in the recovery and purification of biomolecules. ATPF is based on a combination of aqueous two-phase systems (ATPS) and solvent sublation (SS). In this report lipase was recovered and purified from the fermentation broth of Burkholderia cepacia (B. cepacia) using an alcohol/salt ATPF system on a pilot scale. The working parameters of ATPF, including concentration of crude lipase feedstock, types of alcohol and salt, concentrations of alcohol and salt, volumes of buffer solution and alcohol, were investigated for their effects on the partitioning behavior of lipase in ATPF. ATPF comprised of 1-propanol and ammonium sulphate was successfully established for feasible and cost effective separation of B. cepacia ST8 lipase from liquid fermentation broth. The alcohol/salt ATPF system showed a purification factor of 12.2, a separation efficiency of 93% and a selectivity of 40. Furthermore, a comparison has been made between small-scale and large-scale ATPF production. Our results showed that this novel pilot-scale ATPF has the potential to be applied at industrial scale.

Original language	British English
Pages (from-to)	206-213
Number of pages	8
Journal	Separation and Purification Technology
Volume	171
DOIs	https://doi.org/10.1016/j.seppur.2016.07.017
State	Published - 17 Oct 2016

Keywords

Alcohol
Aqueous two-phase flotation
Lipase
Purification
Salt

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10.1016/j.seppur.2016.07.017

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@article{53995019c4f048acba49ebad38b95819,

title = "Pilot-scale aqueous two-phase floatation for direct recovery of lipase derived from Burkholderia cepacia strain ST8",

abstract = "The efficient and economical pilot-scale production of lipase is necessary for meeting the growing demand of lipase especially in bioprocessing industry. Aqueous two-phase flotation (ATPF) is a purification technique that can be applied in the recovery and purification of biomolecules. ATPF is based on a combination of aqueous two-phase systems (ATPS) and solvent sublation (SS). In this report lipase was recovered and purified from the fermentation broth of Burkholderia cepacia (B. cepacia) using an alcohol/salt ATPF system on a pilot scale. The working parameters of ATPF, including concentration of crude lipase feedstock, types of alcohol and salt, concentrations of alcohol and salt, volumes of buffer solution and alcohol, were investigated for their effects on the partitioning behavior of lipase in ATPF. ATPF comprised of 1-propanol and ammonium sulphate was successfully established for feasible and cost effective separation of B. cepacia ST8 lipase from liquid fermentation broth. The alcohol/salt ATPF system showed a purification factor of 12.2, a separation efficiency of 93% and a selectivity of 40. Furthermore, a comparison has been made between small-scale and large-scale ATPF production. Our results showed that this novel pilot-scale ATPF has the potential to be applied at industrial scale.",

keywords = "Alcohol, Aqueous two-phase flotation, Lipase, Purification, Salt",

author = "Preshna Mathiazakan and Shing, {Seah Yieng} and Ying, {Sia Sin} and Kek, {Heng Keng} and Tang, {Malcolm S.Y.} and Show, {Pau Loke} and Ooi, {Chien Wei} and Ling, {Tau Chuan}",

note = "Funding Information: The authors gratefully acknowledge the financial support from Fundamental Research Grant Scheme ( FP005-2013B , FRGS/1/2015/SG05/UNIM/03/1 ), the Ministry of Science and Technology , ( MOSTI 02-02-12-SF0256 ), the Prototype Research Grant Scheme ( PRGS/2/2015/SG05/UNIM/03/1 ), and the University of Malaya ( FP005-2013B , PG037-2013A , RU022E-2014 , RP031B-15AET , PG116-2012B , RU018-2015 ). Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",

year = "2016",

month = oct,

day = "17",

doi = "10.1016/j.seppur.2016.07.017",

language = "British English",

volume = "171",

pages = "206--213",

journal = "Separation and Purification Technology",

issn = "1383-5866",

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T1 - Pilot-scale aqueous two-phase floatation for direct recovery of lipase derived from Burkholderia cepacia strain ST8

AU - Mathiazakan, Preshna

AU - Shing, Seah Yieng

AU - Ying, Sia Sin

AU - Kek, Heng Keng

AU - Tang, Malcolm S.Y.

AU - Show, Pau Loke

AU - Ooi, Chien Wei

AU - Ling, Tau Chuan

N1 - Funding Information: The authors gratefully acknowledge the financial support from Fundamental Research Grant Scheme ( FP005-2013B , FRGS/1/2015/SG05/UNIM/03/1 ), the Ministry of Science and Technology , ( MOSTI 02-02-12-SF0256 ), the Prototype Research Grant Scheme ( PRGS/2/2015/SG05/UNIM/03/1 ), and the University of Malaya ( FP005-2013B , PG037-2013A , RU022E-2014 , RP031B-15AET , PG116-2012B , RU018-2015 ). Publisher Copyright: © 2016 Elsevier B.V.

PY - 2016/10/17

Y1 - 2016/10/17

N2 - The efficient and economical pilot-scale production of lipase is necessary for meeting the growing demand of lipase especially in bioprocessing industry. Aqueous two-phase flotation (ATPF) is a purification technique that can be applied in the recovery and purification of biomolecules. ATPF is based on a combination of aqueous two-phase systems (ATPS) and solvent sublation (SS). In this report lipase was recovered and purified from the fermentation broth of Burkholderia cepacia (B. cepacia) using an alcohol/salt ATPF system on a pilot scale. The working parameters of ATPF, including concentration of crude lipase feedstock, types of alcohol and salt, concentrations of alcohol and salt, volumes of buffer solution and alcohol, were investigated for their effects on the partitioning behavior of lipase in ATPF. ATPF comprised of 1-propanol and ammonium sulphate was successfully established for feasible and cost effective separation of B. cepacia ST8 lipase from liquid fermentation broth. The alcohol/salt ATPF system showed a purification factor of 12.2, a separation efficiency of 93% and a selectivity of 40. Furthermore, a comparison has been made between small-scale and large-scale ATPF production. Our results showed that this novel pilot-scale ATPF has the potential to be applied at industrial scale.

AB - The efficient and economical pilot-scale production of lipase is necessary for meeting the growing demand of lipase especially in bioprocessing industry. Aqueous two-phase flotation (ATPF) is a purification technique that can be applied in the recovery and purification of biomolecules. ATPF is based on a combination of aqueous two-phase systems (ATPS) and solvent sublation (SS). In this report lipase was recovered and purified from the fermentation broth of Burkholderia cepacia (B. cepacia) using an alcohol/salt ATPF system on a pilot scale. The working parameters of ATPF, including concentration of crude lipase feedstock, types of alcohol and salt, concentrations of alcohol and salt, volumes of buffer solution and alcohol, were investigated for their effects on the partitioning behavior of lipase in ATPF. ATPF comprised of 1-propanol and ammonium sulphate was successfully established for feasible and cost effective separation of B. cepacia ST8 lipase from liquid fermentation broth. The alcohol/salt ATPF system showed a purification factor of 12.2, a separation efficiency of 93% and a selectivity of 40. Furthermore, a comparison has been made between small-scale and large-scale ATPF production. Our results showed that this novel pilot-scale ATPF has the potential to be applied at industrial scale.

KW - Alcohol

KW - Aqueous two-phase flotation

KW - Lipase

KW - Purification

KW - Salt

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U2 - 10.1016/j.seppur.2016.07.017

DO - 10.1016/j.seppur.2016.07.017

M3 - Article

AN - SCOPUS:84979266563

SN - 1383-5866

VL - 171

SP - 206

EP - 213

JO - Separation and Purification Technology

JF - Separation and Purification Technology

ER -

Pilot-scale aqueous two-phase floatation for direct recovery of lipase derived from Burkholderia cepacia strain ST8

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Keywords

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