TY - JOUR
T1 - Photostabilization of phycocyanin from Spirulina platensis modified by formaldehyde
AU - Munawaroh, Heli Siti Halimatul
AU - Gumilar, Gun Gun
AU - Alifia, Chindiar Rizka
AU - Marthania, Meganita
AU - Stellasary, Bianca
AU - Yuliani, Galuh
AU - Wulandari, Asri Peni
AU - Kurniawan, Isman
AU - Hidayat, Rahmat
AU - Ningrum, Andriati
AU - Koyande, Apurav Krishna
AU - Show, Pau Loke
N1 - Funding Information:
This research was supported by Universitas Pendidikan Indonesia through Intellectual Copyright-Scheme 2018 and Competitive grant for 2019 , and Directorate General of Science and Technology and Higher Education Resources, Republik of Indonesia through World Class Professor Program-Scheme A 2019 ( T/44/D.2.3/KK.04.05/2019 ).
Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/7
Y1 - 2020/7
N2 - Spirulina platensis contain variety of pigments, such as chlorophylls, carotenoids, and phycocyanin. Phycocyanin (PC) exists in abundance, but due to its instability, the utilization of this pigment is still very limited. In this study, the PC was modified using formaldehyde crosslinks yielding phycocyanin-formaldehyde (PC-F), and its photostability was evaluated. The PC-F formation was designated by the distinctive alterations of the maximum absorption to 611 nm, which was 10 nm blue-shifted than those of the PC. Additionally, the sharp peaks of FTIR spectra at 1636 nm for [sbnd]C[dbnd]O and at 1019 nm for [sbnd]C[sbnd]O[sbnd]C, suggesting the interaction of phycocyanin with formaldehyde. The PC-F showed stabilization improvement up to 1.53-folds after 300 mins of yellow light exposure than those of PC. Contrary to yellow light irradiation, a severe decrease of PC-F absorbance was observed reach to 4.9-folds under UV-B irradiation. The poor stability of PC-F upon white light and UV-A irradiation were indicated by the decline of PC-F absorbance up to 1.72 and 1.80, respectively. Moreover, the present study suggests that the modification of phycocyanin by formaldehyde crosslink can increase photostability upon yellow light irradiation.
AB - Spirulina platensis contain variety of pigments, such as chlorophylls, carotenoids, and phycocyanin. Phycocyanin (PC) exists in abundance, but due to its instability, the utilization of this pigment is still very limited. In this study, the PC was modified using formaldehyde crosslinks yielding phycocyanin-formaldehyde (PC-F), and its photostability was evaluated. The PC-F formation was designated by the distinctive alterations of the maximum absorption to 611 nm, which was 10 nm blue-shifted than those of the PC. Additionally, the sharp peaks of FTIR spectra at 1636 nm for [sbnd]C[dbnd]O and at 1019 nm for [sbnd]C[sbnd]O[sbnd]C, suggesting the interaction of phycocyanin with formaldehyde. The PC-F showed stabilization improvement up to 1.53-folds after 300 mins of yellow light exposure than those of PC. Contrary to yellow light irradiation, a severe decrease of PC-F absorbance was observed reach to 4.9-folds under UV-B irradiation. The poor stability of PC-F upon white light and UV-A irradiation were indicated by the decline of PC-F absorbance up to 1.72 and 1.80, respectively. Moreover, the present study suggests that the modification of phycocyanin by formaldehyde crosslink can increase photostability upon yellow light irradiation.
KW - Formaldehyde crosslink
KW - Photostability
KW - Phycocyanin modified
KW - Spirulina platensis
KW - Thermal stability
UR - http://www.scopus.com/inward/record.url?scp=85084480134&partnerID=8YFLogxK
U2 - 10.1016/j.procbio.2020.04.021
DO - 10.1016/j.procbio.2020.04.021
M3 - Article
AN - SCOPUS:85084480134
SN - 1359-5113
VL - 94
SP - 297
EP - 304
JO - Process Biochemistry
JF - Process Biochemistry
ER -