pH responsive adhesion of phospholipid vesicle on poly(acrylic acid) cushion grafted to poly(ethylene terephthalate) surface

Ning Fang; Jin Tan Wee; Kam W. Leong; Hai Quan Mao; Vincent Chan

doi:10.1016/j.colsurfb.2004.11.002

pH responsive adhesion of phospholipid vesicle on poly(acrylic acid) cushion grafted to poly(ethylene terephthalate) surface

Ning Fang, Jin Tan Wee, Kam W. Leong, Hai Quan Mao, Vincent Chan

Biomedical Engineering & Biotechnology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Polymer-supported lipid bilayer is a key enabling technology for the design and fabrication of novel biomimetic devices. To date, the physical driving force underlying the formation of polymer-supported lipid bilayer remains to be determined. In this study, the interaction between dipalmitoylphosphocholine (DPPC) vesicle and poly(ethylene terephthalate) [PET] surface with or without grafted poly(acrylic acid) [PAA] layer is examined with several biophysical techniques. First, vesicle deformation analysis shows that the geometry of adherent vesicle on either plain PET or PAA-grafted PET surface is best described by a truncated sphere model. At neutral pH, the degree of deformation and adhesion energy are unaltered by the grafted polymerization of acrylic acid on PET surface. Interestingly, the average magnitude of adhesion energy is increased by 185% and -43% on PAA-grated PET and plain PET surface, respectively, towards an increase of pH at room temperature. Our results demonstrate the possibility of tuning the adhesive interaction between vesicle and polymer cushion through the control of polyelectrolyte ionization on the solid support.

Original language	British English
Pages (from-to)	245-252
Number of pages	8
Journal	Colloids and Surfaces B: Biointerfaces
Volume	42
Issue number	3-4
DOIs	https://doi.org/10.1016/j.colsurfb.2004.11.002
State	Published - 25 May 2005

Keywords

Dipalmitoylphosphocholine
Phospholipid vesicle
Poly(ethylene terephthalate)

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10.1016/j.colsurfb.2004.11.002

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@article{38d286901cef4295b843ffd5a356138f,

title = "pH responsive adhesion of phospholipid vesicle on poly(acrylic acid) cushion grafted to poly(ethylene terephthalate) surface",

abstract = "Polymer-supported lipid bilayer is a key enabling technology for the design and fabrication of novel biomimetic devices. To date, the physical driving force underlying the formation of polymer-supported lipid bilayer remains to be determined. In this study, the interaction between dipalmitoylphosphocholine (DPPC) vesicle and poly(ethylene terephthalate) [PET] surface with or without grafted poly(acrylic acid) [PAA] layer is examined with several biophysical techniques. First, vesicle deformation analysis shows that the geometry of adherent vesicle on either plain PET or PAA-grafted PET surface is best described by a truncated sphere model. At neutral pH, the degree of deformation and adhesion energy are unaltered by the grafted polymerization of acrylic acid on PET surface. Interestingly, the average magnitude of adhesion energy is increased by 185% and -43% on PAA-grated PET and plain PET surface, respectively, towards an increase of pH at room temperature. Our results demonstrate the possibility of tuning the adhesive interaction between vesicle and polymer cushion through the control of polyelectrolyte ionization on the solid support.",

keywords = "Dipalmitoylphosphocholine, Phospholipid vesicle, Poly(ethylene terephthalate)",

author = "Ning Fang and Wee, {Jin Tan} and Leong, {Kam W.} and Mao, {Hai Quan} and Vincent Chan",

note = "Funding Information: VC and NF were supported by a Tissue Engineering Initiative of Professor Lim Mong King (NTU) and NTU AcRF 00/15. CL, HQM and KWL were partially supported by NIH grant (5P01CA79862-020003) and A*STAR (Singapore). ",

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T1 - pH responsive adhesion of phospholipid vesicle on poly(acrylic acid) cushion grafted to poly(ethylene terephthalate) surface

AU - Fang, Ning

AU - Wee, Jin Tan

AU - Leong, Kam W.

AU - Mao, Hai Quan

AU - Chan, Vincent

N1 - Funding Information: VC and NF were supported by a Tissue Engineering Initiative of Professor Lim Mong King (NTU) and NTU AcRF 00/15. CL, HQM and KWL were partially supported by NIH grant (5P01CA79862-020003) and A*STAR (Singapore).

PY - 2005/5/25

Y1 - 2005/5/25

N2 - Polymer-supported lipid bilayer is a key enabling technology for the design and fabrication of novel biomimetic devices. To date, the physical driving force underlying the formation of polymer-supported lipid bilayer remains to be determined. In this study, the interaction between dipalmitoylphosphocholine (DPPC) vesicle and poly(ethylene terephthalate) [PET] surface with or without grafted poly(acrylic acid) [PAA] layer is examined with several biophysical techniques. First, vesicle deformation analysis shows that the geometry of adherent vesicle on either plain PET or PAA-grafted PET surface is best described by a truncated sphere model. At neutral pH, the degree of deformation and adhesion energy are unaltered by the grafted polymerization of acrylic acid on PET surface. Interestingly, the average magnitude of adhesion energy is increased by 185% and -43% on PAA-grated PET and plain PET surface, respectively, towards an increase of pH at room temperature. Our results demonstrate the possibility of tuning the adhesive interaction between vesicle and polymer cushion through the control of polyelectrolyte ionization on the solid support.

AB - Polymer-supported lipid bilayer is a key enabling technology for the design and fabrication of novel biomimetic devices. To date, the physical driving force underlying the formation of polymer-supported lipid bilayer remains to be determined. In this study, the interaction between dipalmitoylphosphocholine (DPPC) vesicle and poly(ethylene terephthalate) [PET] surface with or without grafted poly(acrylic acid) [PAA] layer is examined with several biophysical techniques. First, vesicle deformation analysis shows that the geometry of adherent vesicle on either plain PET or PAA-grafted PET surface is best described by a truncated sphere model. At neutral pH, the degree of deformation and adhesion energy are unaltered by the grafted polymerization of acrylic acid on PET surface. Interestingly, the average magnitude of adhesion energy is increased by 185% and -43% on PAA-grated PET and plain PET surface, respectively, towards an increase of pH at room temperature. Our results demonstrate the possibility of tuning the adhesive interaction between vesicle and polymer cushion through the control of polyelectrolyte ionization on the solid support.

KW - Dipalmitoylphosphocholine

KW - Phospholipid vesicle

KW - Poly(ethylene terephthalate)

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pH responsive adhesion of phospholipid vesicle on poly(acrylic acid) cushion grafted to poly(ethylene terephthalate) surface

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