Perforin deficiency attenuates collagen-induced arthritis.

Kristin Bauer, Annika Knipper, Tu Rapp Hoang, Dirk Koczan, Hans Jürgen Kreutzer, Horst Nizze, Eilhard Mix, Hans Juergen Thiesen, Rikard Holmdahl, Saleh M. Ibrahim

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Collagen-induced arthritis (CIA), an approved animal model for rheumatoid arthritis, is thought to be a T cell-dependent disease. There is evidence that CD8+ T cells are a major subset controlling the pathogenesis of CIA. They probably contribute to certain features of disease, namely tissue destruction and synovial hyperplasia. In this study we examined the role of perforin (pfp), a key molecule of the cytotoxic death pathway that is expressed mainly in CD8+ T cells, for the pathogenesis of CIA. We generated DBA/1J mice suffering from mutations of the pfp molecule, DBA/1J-pfp-/-, and studied their susceptibility to arthritis. As a result, pfp-deficient mice showed a reduced incidence (DBA/1J-pfp+/+, 64%; DBA/1J-pfp-/-, 54%), a slightly delayed onset (onset of disease: DBA/1J-pfp+/+, 53 +/- 3.6; DBA/1J-pfp-/-, 59 +/- 4.9 (mean +/- SEM), and milder form of the disease (maximum disease score: DBA/1J-pfp+/+, 7.3 +/- 1.1; DBA/1J-pfp-/-, 3.4 +/- 1.4 (mean +/- SEM); P < 0.05). Concomitantly, peripheral T cell proliferation in response to the specific antigen bovine collagen II was increased in pfp-/- mice compared with pfp+/+ mice, arguing for an impaired killing of autoreactive T cells caused by pfp deficiency. Thus, pfp-mediated cytotoxicity is involved in the initiation of tissue damage in arthritis, but pfp-independent cytotoxic death pathways might also contribute to CIA.

Original languageBritish English
Pages (from-to)R877-884
JournalArthritis Research and Therapy
Issue number4
StatePublished - 2005


Dive into the research topics of 'Perforin deficiency attenuates collagen-induced arthritis.'. Together they form a unique fingerprint.

Cite this