Overproduction of lipoxygenase from Pseudomonas aeruginosa in Escherichia coli by auto-induction expression and its application in triphenylmethane dyes degradation

Jing Lu, Chong Zhang, Hui Yi Leong, Pau Loke Show, Fengxia Lu, Zhaoxin Lu

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

In this study, the bacterial lipoxygenase (LOX) gene from Pseudomonas aeruginosa ATCC27853 (pse-LOX) was cloned, sequenced and heterologous expressed in Escherichia coli by auto-induction expression strategy. Production of the recombinant pse-LOX (pse-rLOX) gene up to 23,850 U/mL (264 mg pure protein/L bacterial culture fluid) was observed in the end of this process. To the best of our knowledge, this is the first attempt to manipulate LOX heterologous expression process using auto-induction expression approach, and it is the highest production of recombinant LOX compared with other reports. Subsequently, the resulted pse-rLOX was proved to efficiently degrade triphenylmethane dyes such as malachite green, brilliant green and aniline blue. Generally, an overproduction of the LOX from P. aeruginosa was observed in E. coli, and this recombinant gene is a potential candidate as biocatalyst for triphenylmethane dyes decolorization.

Original languageBritish English
Pages (from-to)327-332
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume129
Issue number3
DOIs
StatePublished - Mar 2020

Keywords

  • Auto-induction expression
  • Biocatalyst
  • Decolorization
  • Escherichia coli
  • Pseudomonas aeruginosa
  • Recombinant lipoxygenase
  • Triphenylmethane dyes

Fingerprint

Dive into the research topics of 'Overproduction of lipoxygenase from Pseudomonas aeruginosa in Escherichia coli by auto-induction expression and its application in triphenylmethane dyes degradation'. Together they form a unique fingerprint.

Cite this