Abstract
An alternative phase-forming component, made using alcohols (e.g., methanol, ethanol, 1-propanol, and 2-propanol), has overcome the restrictions of conventional polymer-based liquid biphasic systems (LBSs). The advantages of alcohol-based LBSs are a low-cost phase-forming component, the easy recovery of biomolecules (by evaporation of the alcohol), and a component that can be recycled and reused for the next extraction and purification process. The basic principles of alcohol-based LBSs for the extraction of various biomolecules are discussed. The various key parameters such as type and concentration of alcohol and salt, temperature, pH, and the addition of neutral salt are comprehensively evaluated. In addition, the application of alcohol-based LBSs for the extraction of various biomolecules from complex crude feedstock and the experimental research utilizing alcohol-based LBSs are summarized. This chapter provides insight for researchers who are dealing with separation and purification technology.
| Original language | British English |
|---|---|
| Title of host publication | Liquid Biphasic System |
| Subtitle of host publication | Fundamentals and Applications in Bioseparation Technology |
| Publisher | Elsevier |
| Pages | 39-50 |
| Number of pages | 12 |
| ISBN (Electronic) | 9780128199862 |
| DOIs | |
| State | Published - 1 Jan 2020 |
Keywords
- Biomolecules
- Extraction efficiency
- Liquid biphasic system
- Organic-based
- Purification