TY - JOUR
T1 - Optimization of Gold Nanoparticles for Efficient Delivery of Catalase to Macrophages for Alleviating Inflammation
AU - Ba Fakih, Fatima
AU - Shanti, Aya
AU - Stefanini, Cesare
AU - Lee, Sungmun
N1 - Funding Information:
C.S. and S.L. were involved in planning the research. S.L. supervised F.B.F. and A.S. to perform all experiments. F.B.F. synthesized different types of AuNPs and characterized all catalase-conjugated AuNP. F.B.F. collected all cell culture data with the help of A.S. F.B.F., A.S., and S.L. participated in writing the manuscript based on the input of all authors. C.S. also supported this project financially.
Publisher Copyright:
Copyright © 2020 American Chemical Society.
PY - 2020/9/25
Y1 - 2020/9/25
N2 - Inflammation-associated diseases are the leading causes of death worldwide. They are associated with oxidative stress that is the overexpression of reactive oxygen species, such as hydrogen peroxide (H2O2) secreted by macrophages. In this study, gold nanoparticles (AuNPs) were used to deliver catalase that metabolizes H2O2 to macrophages. Ten different types were tested, including five spherical AuNPs with various sizes (50, 80, 100, 200, and 400 nm) and five shapes of AuNPs (cube, hexagon, bean, rod, and tetrapod). After catalase conjugation to AuNPs, optimum size and shape were selected by the highest uptake of AuNPs by macrophages. More than 80% macrophages were infected by AuNPs when treated with spherical AuNPs (100, 200, or 400 nm) of cube or bean shape. All of them reduced the exogenous H2O2 to match the control; however, bean AuNPs were superior in cell viability under H2O2 treatment. Based on the results, we anticipate numerous applications of bean AuNPs in the treatment of inflammation-associated diseases.
AB - Inflammation-associated diseases are the leading causes of death worldwide. They are associated with oxidative stress that is the overexpression of reactive oxygen species, such as hydrogen peroxide (H2O2) secreted by macrophages. In this study, gold nanoparticles (AuNPs) were used to deliver catalase that metabolizes H2O2 to macrophages. Ten different types were tested, including five spherical AuNPs with various sizes (50, 80, 100, 200, and 400 nm) and five shapes of AuNPs (cube, hexagon, bean, rod, and tetrapod). After catalase conjugation to AuNPs, optimum size and shape were selected by the highest uptake of AuNPs by macrophages. More than 80% macrophages were infected by AuNPs when treated with spherical AuNPs (100, 200, or 400 nm) of cube or bean shape. All of them reduced the exogenous H2O2 to match the control; however, bean AuNPs were superior in cell viability under H2O2 treatment. Based on the results, we anticipate numerous applications of bean AuNPs in the treatment of inflammation-associated diseases.
KW - catalase
KW - drug delivery
KW - gold nanoparticles
KW - inflammation-associated diseases
KW - macrophages
KW - reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=85094947546&partnerID=8YFLogxK
U2 - 10.1021/acsanm.0c02234
DO - 10.1021/acsanm.0c02234
M3 - Article
AN - SCOPUS:85094947546
SN - 2574-0970
VL - 3
SP - 9510
EP - 9519
JO - ACS Applied Nano Materials
JF - ACS Applied Nano Materials
IS - 9
ER -