TY - GEN
T1 - Optical Fibre FPI End-Tip based Sensor for Protein Aggregation Detection
AU - Fatima Domingues, M.
AU - Direito, Ines
AU - Sousa, Carolina
AU - Radwan, Ayman
AU - Antunes, Paulo
AU - Andre, Paulo
AU - Helguero, Luisa
AU - Alberto, Nelia
N1 - Funding Information:
This work was funded by FCT/MCTES and FCT/MEC through national funds and when applicable co-funded EU funds under the projects, UIDB/50008/2020- UIDP/50008/2020, UIDB/50025/2020-UIDP/50025/2020, and UIDB/04501/2020 and UIDP/04501/2020. This work was also supported by the Scientific Employment Stimulus - Institutional Call CEECINST/00026/2018 and the SR&TD Integrated Programmes "pAGE-Protein aggregation Across the Lifespan" (CENTRO-01-0145-FEDER-000003), d by MEDISIS (CENTRO-01-0246- FEDER-000018). The funds of the European Regional Development Fund (FEDER), through the Regional Operational Programme of Lisbon (POR LISBOA 2020) and the Regional Operational Programme of Centre (CENTRO 2020) of the Portugal 2020 framework [Project Safe-Home with Nr. 072082 (CENTRO- 01-0247-FEDER-072082)] are also acknowledged. M. F. Domingues and N. Alberto acknowledge the scientific actions REACT and PREDICT, funded by FCT/MEC through national funds and when applicable co-funded by FEDER - PT2020 partnership agreement under the project UID/EEA/50008/2019. Inês Direito acknowledges the FCT PhD grants COVID/BD/151682/2022 and SFRH/BD/123821/2016.
Funding Information:
This work was funded by FCT/MCTES and FCT/MEC through national funds and when applicable co-funded EU funds under the projects, UIDB/50008/2020-UIDP/50008/2020, UIDB/50025/2020-UIDP/50025/2020, and UIDB/04501/2020 and UIDP/04501/2020. This work was also supported by the Scientific Employment Stimulus - Institutional Call CEECINST/00026/2018 and the SR&TD Integrated Programmes “pAGE—Protein aggregation Across the Lifespan” (CENTRO-01-0145-
Funding Information:
FEDER-000003), d by MEDISIS (CENTRO-01-0246-FEDER-000018). The funds of the European Regional Development Fund (FEDER), through the Regional Operational Programme of Lisbon (POR LISBOA 2020) and the Regional Operational Programme of Centre (CENTRO 2020) of the Portugal 2020 framework [Project Safe-Home with Nr. 072082 (CENTRO-01-0247-FEDER-072082)] are also acknowledged. M. F. Domingues and N. Alberto acknowledge the scientific actions REACT and PREDICT, funded by FCT/MEC through national funds and when applicable co-funded by FEDER – PT2020 partnership agreement under the project UID/EEA/50008/2019. Inês Direito acknowledges the FCT PhD grants COVID/BD/151682/2022 and SFRH/BD/123821/2016.
Publisher Copyright:
© 2022 IEEE.
PY - 2022
Y1 - 2022
N2 - The detection of protein aggregates and its concentration, plays a relevant role in several fronts, namely at the detection of neurodegenerative diseases such as Alzheimer's, and the assessment of cellular stress linked to cell death. Evaluation of protein aggregation is an essential step of quality control in all stages of biopharmaceutical synthesis and transport, in order to guarantee the activity and desired immunogenicity of their products. The detection of protein aggregates often requires the use of expensive and complex techniques. Here we propose a fast and easy implementation solution, based on an optical fibre Fabry-Perot Interferometer (FPI) end tip resonator. The solution proposed revealed to be effective on the detection of protein aggregates, providing similar results as the ones obtained using a commercial spectrometer for fluorescence detection.
AB - The detection of protein aggregates and its concentration, plays a relevant role in several fronts, namely at the detection of neurodegenerative diseases such as Alzheimer's, and the assessment of cellular stress linked to cell death. Evaluation of protein aggregation is an essential step of quality control in all stages of biopharmaceutical synthesis and transport, in order to guarantee the activity and desired immunogenicity of their products. The detection of protein aggregates often requires the use of expensive and complex techniques. Here we propose a fast and easy implementation solution, based on an optical fibre Fabry-Perot Interferometer (FPI) end tip resonator. The solution proposed revealed to be effective on the detection of protein aggregates, providing similar results as the ones obtained using a commercial spectrometer for fluorescence detection.
KW - biomedical optical probes
KW - Fabry-Perot cavities
KW - photonic sensors
KW - Protein aggregation detection
KW - resonator end-tip probes
UR - http://www.scopus.com/inward/record.url?scp=85146264485&partnerID=8YFLogxK
U2 - 10.1109/HealthCom54947.2022.9982774
DO - 10.1109/HealthCom54947.2022.9982774
M3 - Conference contribution
AN - SCOPUS:85146264485
T3 - 2022 IEEE International Conference on E-Health Networking, Application and Services, HealthCom 2022
SP - 129
EP - 134
BT - 2022 IEEE International Conference on E-Health Networking, Application and Services, HealthCom 2022
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2022 IEEE International Conference on E-health Networking, Application and Services, HealthCom 2022
Y2 - 17 October 2022 through 19 October 2022
ER -