TY - JOUR
T1 - One-Pot Ionic Liquid-Mediated Bioprocess for Pretreatment and Enzymatic Hydrolysis of Rice Straw with Ionic Liquid-Tolerance Bacterial Cellulase
AU - Sriariyanun, Malinee
AU - Kitiborwornkul, Nichaphat
AU - Tantayotai, Prapakorn
AU - Rattanaporn, Kittipong
AU - Show, Pau Loke
N1 - Funding Information:
Funding: This research was funded by King Mongkut’s University of Technology, North Bangkok, grant number KMUTNB-FF-65-37 and Srinakharinwirot University, grant number 583/2564, and The APC was funded by King Mongkut’s University of Technology, North Bangkok.
Funding Information:
This research was funded by King Mongkut?s University of Technology, North Bangkok, grant number KMUTNB-FF-65-37 and Srinakharinwirot University, grant number 583/2564, and The APC was funded by King Mongkut?s University of Technology, North Bangkok.
Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/1
Y1 - 2022/1
N2 - Ionic liquid (IL) pretreatment of lignocellulose is an efficient method for the enhancement of enzymatic saccharification. However, the remaining residues of ILs deactivate cellulase, therefore making intensive biomass washing after pretreatment necessary. This study aimed to develop the one-pot process combining IL pretreatment and enzymatic saccharification by using low-toxic choline acetate ([Ch][OAc]) and IL-tolerant bacterial cellulases. Crude cellulases produced from saline soil inhabited Bacillus sp. CBD2 and Brevibacillus sp. CBD3 were tested under the influence of 0.5–2.0 M [Ch][OAc], which showed that their activities retained at more than 95%. However, [Ch][OAc] had toxicity to CBD2 and CBD3 cultures, in which only 32.85% and 12.88% were alive at 0.5 M [Ch][OAc]. Based on the specific enzyme activities, the sugar amounts produced from one-pot processes using 1 mg of CBD2 and CBD3 were higher than that of Celluclast 1.5 L by 2.0 and 4.5 times, respectively, suggesting their potential for further application in the biorefining process of value-added products.
AB - Ionic liquid (IL) pretreatment of lignocellulose is an efficient method for the enhancement of enzymatic saccharification. However, the remaining residues of ILs deactivate cellulase, therefore making intensive biomass washing after pretreatment necessary. This study aimed to develop the one-pot process combining IL pretreatment and enzymatic saccharification by using low-toxic choline acetate ([Ch][OAc]) and IL-tolerant bacterial cellulases. Crude cellulases produced from saline soil inhabited Bacillus sp. CBD2 and Brevibacillus sp. CBD3 were tested under the influence of 0.5–2.0 M [Ch][OAc], which showed that their activities retained at more than 95%. However, [Ch][OAc] had toxicity to CBD2 and CBD3 cultures, in which only 32.85% and 12.88% were alive at 0.5 M [Ch][OAc]. Based on the specific enzyme activities, the sugar amounts produced from one-pot processes using 1 mg of CBD2 and CBD3 were higher than that of Celluclast 1.5 L by 2.0 and 4.5 times, respectively, suggesting their potential for further application in the biorefining process of value-added products.
KW - Biorefinery
KW - Cellulase
KW - Enzymatic hydrolysis
KW - Ionic liquid pretreatment
KW - Lignocellulose biomass
KW - One-pot process
UR - http://www.scopus.com/inward/record.url?scp=85122301102&partnerID=8YFLogxK
U2 - 10.3390/bioengineering9010017
DO - 10.3390/bioengineering9010017
M3 - Article
AN - SCOPUS:85122301102
SN - 2306-5354
VL - 9
JO - Bioengineering
JF - Bioengineering
IS - 1
M1 - 17
ER -