@article{c862a84c5a3e4e16add3dc03337aa36c,
title = "NuMA1 promotes axon initial segment assembly through inhibition of endocytosis",
abstract = "Axon initial segments (AISs) initiate action potentials and regulate the trafficking of vesicles between somatodendritic and axonal compartments. However, the mechanisms controlling AIS assembly remain poorly defined. We performed differential proteomics and found nuclear mitotic apparatus protein 1 (NuMA1) is downregulated in AIS-deficient neonatal mouse brains and neurons. NuMA1 is transiently located at the AIS during development where it interacts with the scaffolding protein 4.1B and the dynein regulator lissencephaly 1 (Lis1). Silencing NuMA1 or protein 4.1B by shRNA disrupts AIS assembly, but not maintenance. Silencing Lis1 or overexpressing NuMA1 during AIS assembly increased the density of AIS proteins, including ankyrinG and neurofascin-186 (NF186). NuMA1 inhibits the endocytosis of AIS NF186 by impeding Lis1's interaction with doublecortin, a potent facilitator of NF186 endocytosis. Our results indicate the transient expression and AIS localization of NuMA1 stabilizes the developing AIS by inhibiting endocytosis and removal of AIS proteins.",
author = "Tomohiro Torii and Yuki Ogawa and Liu, \{Cheng Hsin\} and Ho, \{Tammy Szu Yu\} and Hamdan Hamdan and Wang, \{Chih Chuan\} and Oses-Prieto, \{Juan A.\} and Burlingame, \{Alma L.\} and Rasband, \{Matthew N.\}",
note = "Funding Information: The work reported here was supported by the following research grants: National Institutes of Health NS044916 and NS069688 (to M.N. Rasband); and by the Dr. Miriam and Sheldon G. Adelson Medical Research Foundation (to A.L. Burlingame and M.N. Rasband). Publisher Copyright: {\textcopyright} 2019 Torii et al. This article is distributed under the terms of an Attribution-Noncommercial-Share Alike-No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution-Noncommercial-Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).",
year = "2020",
month = feb,
day = "3",
doi = "10.1083/jcb.201907048",
language = "British English",
volume = "219",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "2",
}