Non-target inhibition of antioxidant enzymes in honey bees (A. mellifera and a. florea) upon pesticide exposure

Amal Ahmed Berjawi; Syed Salman Ashraf; Mohammad Ali Al-Deeb

doi:10.3844/ojbsci.2020.57.65

Non-target inhibition of antioxidant enzymes in honey bees (A. mellifera and a. florea) upon pesticide exposure

Amal Ahmed Berjawi
, Syed Salman Ashraf
, Mohammad Ali Al-Deeb

Chemistry

United Arab Emirates University

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Understanding how different insecticides influence bee health is very essential. The aim of this work was to evaluate enzyme kinetics and inhibition by insecticides in Apis mellifera and Apis florea. Tested enzymes showed variable affinity to substrates. In A. florea, glutathione S-transferase (GST) showed significantly higher affinity for its 1-Chloro-2,4-Dinitrobenzene (CDNB) substrate than in A. mellifera. Carbosulfan caused the highest inhibition to acetylcholinesterase (AChE) in both tested species in comparison with chlorpyrifos, dimethoate and pirimiphos-methyl. Our results show that exposure to insecticides is harmful to honey bees and results in negative effects on enzyme activity.

Original language	British English
Pages (from-to)	57-65
Number of pages	9
Journal	OnLine Journal of Biological Sciences
Volume	20
Issue number	2
DOIs	https://doi.org/10.3844/ojbsci.2020.57.65
State	Published - 2020

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Apis florea
Apis mellifera
Detoxifying Enzymes
Enzyme Kinetics
Insecticide

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title = "Non-target inhibition of antioxidant enzymes in honey bees (A. mellifera and a. florea) upon pesticide exposure",

abstract = "Understanding how different insecticides influence bee health is very essential. The aim of this work was to evaluate enzyme kinetics and inhibition by insecticides in Apis mellifera and Apis florea. Tested enzymes showed variable affinity to substrates. In A. florea, glutathione S-transferase (GST) showed significantly higher affinity for its 1-Chloro-2,4-Dinitrobenzene (CDNB) substrate than in A. mellifera. Carbosulfan caused the highest inhibition to acetylcholinesterase (AChE) in both tested species in comparison with chlorpyrifos, dimethoate and pirimiphos-methyl. Our results show that exposure to insecticides is harmful to honey bees and results in negative effects on enzyme activity.",

keywords = "Apis florea, Apis mellifera, Detoxifying Enzymes, Enzyme Kinetics, Insecticide",

author = "Berjawi, \{Amal Ahmed\} and Ashraf, \{Syed Salman\} and Al-Deeb, \{Mohammad Ali\}",

note = "Funding Information: The authors thank the Agriculture Research Stations (Kuwaitat), Abu Dhabi Food Control Authority (ADFCA), Al-Ain, UAE, for providing honey bees (A. mellifera). In addition, the authors thank Mr. Ahmed Mahmoud Rajab for providing expert insights on honey bees and beekeeping. Publisher Copyright: {\textcopyright} 2020 Amal Ahmed Berjawi, Syed Salman Ashraf and Mohammad Ali Al-Deeb.",

year = "2020",

doi = "10.3844/ojbsci.2020.57.65",

language = "British English",

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TY - JOUR

T1 - Non-target inhibition of antioxidant enzymes in honey bees (A. mellifera and a. florea) upon pesticide exposure

AU - Berjawi, Amal Ahmed

AU - Ashraf, Syed Salman

AU - Al-Deeb, Mohammad Ali

N1 - Funding Information: The authors thank the Agriculture Research Stations (Kuwaitat), Abu Dhabi Food Control Authority (ADFCA), Al-Ain, UAE, for providing honey bees (A. mellifera). In addition, the authors thank Mr. Ahmed Mahmoud Rajab for providing expert insights on honey bees and beekeeping. Publisher Copyright: © 2020 Amal Ahmed Berjawi, Syed Salman Ashraf and Mohammad Ali Al-Deeb.

PY - 2020

Y1 - 2020

N2 - Understanding how different insecticides influence bee health is very essential. The aim of this work was to evaluate enzyme kinetics and inhibition by insecticides in Apis mellifera and Apis florea. Tested enzymes showed variable affinity to substrates. In A. florea, glutathione S-transferase (GST) showed significantly higher affinity for its 1-Chloro-2,4-Dinitrobenzene (CDNB) substrate than in A. mellifera. Carbosulfan caused the highest inhibition to acetylcholinesterase (AChE) in both tested species in comparison with chlorpyrifos, dimethoate and pirimiphos-methyl. Our results show that exposure to insecticides is harmful to honey bees and results in negative effects on enzyme activity.

AB - Understanding how different insecticides influence bee health is very essential. The aim of this work was to evaluate enzyme kinetics and inhibition by insecticides in Apis mellifera and Apis florea. Tested enzymes showed variable affinity to substrates. In A. florea, glutathione S-transferase (GST) showed significantly higher affinity for its 1-Chloro-2,4-Dinitrobenzene (CDNB) substrate than in A. mellifera. Carbosulfan caused the highest inhibition to acetylcholinesterase (AChE) in both tested species in comparison with chlorpyrifos, dimethoate and pirimiphos-methyl. Our results show that exposure to insecticides is harmful to honey bees and results in negative effects on enzyme activity.

KW - Apis florea

KW - Apis mellifera

KW - Detoxifying Enzymes

KW - Enzyme Kinetics

KW - Insecticide

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DO - 10.3844/ojbsci.2020.57.65

M3 - Article

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SN - 1608-4217

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JF - OnLine Journal of Biological Sciences

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ER -

Non-target inhibition of antioxidant enzymes in honey bees (A. mellifera and a. florea) upon pesticide exposure

Abstract

UN SDGs

Keywords

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