Molecular decoration of ceramic supports for highly effective enzyme immobilization-material approach

Joanna Kujawa, Marta Głodek, Izabela Koter, Borys Ośmialowski, Katarzyna Knozowska, Samer Al-Gharabli, Ludovic F. Dumée, Wojciech Kujawski

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

A highly effective method was developed to functionalize ceramic supports (Al2O3 powders and membranes) using newly synthesized spacer molecules. The functionalized materials were subsequently utilized for Candida antarctica lipase B enzyme immobilization. The objective is to systematically evaluate the impact of various spacer molecules grafted onto the alumina materials will affect both the immobilization of the enzymes and specific material surface properties, critical to enzymatic reactors performance. The enzyme loading was significantly improved for the supports modified with shorter spacer molecules, which possessed higher grafting effectiveness on the order of 90%. The specific enzyme activity was found to be much higher for samples functionalized with longer modifiers yielding excellent enantioselectivity > 97%. However, the enantiomeric ratio of the immobilized lipase was slightly lower in the case of shorter spacer molecules.

Original languageBritish English
Article number201
Pages (from-to)1-18
Number of pages18
JournalMaterials
Volume14
Issue number1
DOIs
StatePublished - Jan 2021

Keywords

  • Alumina oxide
  • Candida antarctica lipase B
  • Ceramic membranes
  • Enzyme immobilization
  • Organic spacer
  • Surface modification

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