TY - JOUR
T1 - Microfluidic Assay To Study the Combinatorial Impact of Substrate Properties on Mesenchymal Stem Cell Migration
AU - Menon, Nishanth V.
AU - Chuah, Yon Jin
AU - Phey, Samantha
AU - Zhang, Ying
AU - Wu, Yingnan
AU - Chan, Vincent
AU - Kang, Yuejun
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2015/8/12
Y1 - 2015/8/12
N2 - As an alternative to complex and costly in vivo models, microfluidic in vitro models are being widely used to study various physiological phenomena. It is of particular interest to study cell migration in a controlled microenvironment because of its vital role in a large number of physiological processes, such as wound healing, disease progression, and tissue regeneration. Cell migration has been shown to be affected by variations in the biochemical and physical properties of the extracellular matrix (ECM). To study the combinatorial impact of the ECM physical properties on cell migration, we have developed a microfluidic assay to induce migration of human bone marrow derived mesenchymal stem cells (hBMSCs) on polydimethylsiloxane (PDMS) substrates with varying combinatorial properties (hydrophobicity, stiffness, and roughness). The results show that although the initial cell adhesion and viability appear similar on all PDMS samples, the cell spreading and migration are enhanced on PDMS samples exhibiting intermediate levels of hydrophobicity, stiffness, and roughness. This study suggests that there is a particular range of substrate properties for optimal cell spreading and migration. The influence of substrate properties on hBMSC migration can help understand the physical cues that affect cell migration, which may facilitate the development of optimized engineered scaffolds with desired properties for tissue regeneration applications.
AB - As an alternative to complex and costly in vivo models, microfluidic in vitro models are being widely used to study various physiological phenomena. It is of particular interest to study cell migration in a controlled microenvironment because of its vital role in a large number of physiological processes, such as wound healing, disease progression, and tissue regeneration. Cell migration has been shown to be affected by variations in the biochemical and physical properties of the extracellular matrix (ECM). To study the combinatorial impact of the ECM physical properties on cell migration, we have developed a microfluidic assay to induce migration of human bone marrow derived mesenchymal stem cells (hBMSCs) on polydimethylsiloxane (PDMS) substrates with varying combinatorial properties (hydrophobicity, stiffness, and roughness). The results show that although the initial cell adhesion and viability appear similar on all PDMS samples, the cell spreading and migration are enhanced on PDMS samples exhibiting intermediate levels of hydrophobicity, stiffness, and roughness. This study suggests that there is a particular range of substrate properties for optimal cell spreading and migration. The influence of substrate properties on hBMSC migration can help understand the physical cues that affect cell migration, which may facilitate the development of optimized engineered scaffolds with desired properties for tissue regeneration applications.
KW - cell migration and spreading
KW - extracellular matrix
KW - human-bone-marrow-derived mesenchymal stem cells
KW - roughness
KW - stiffness
KW - substratum properties
KW - wettability
UR - http://www.scopus.com/inward/record.url?scp=84939158725&partnerID=8YFLogxK
U2 - 10.1021/acsami.5b03753
DO - 10.1021/acsami.5b03753
M3 - Article
C2 - 26186177
AN - SCOPUS:84939158725
SN - 1944-8244
VL - 7
SP - 17095
EP - 17103
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 31
ER -