Manipulation of herpes simplex virus type 1 by dielectrophoresis

Michael P. Hughes; Hywel Morgan; Frazer J. Rixon; Julian P.H. Burt; Ronald Pethig

doi:10.1016/S0304-4165(98)00058-0

Manipulation of herpes simplex virus type 1 by dielectrophoresis

Michael P. Hughes, Hywel Morgan, Frazer J. Rixon, Julian P.H. Burt, Ronald Pethig

Biomedical Engineering & Biotechnology

Research output: Contribution to journal › Article › peer-review

155 Scopus citations

Abstract

The frequency-dependent dielectrophoretic behaviour of an enveloped mammalian virus, herpes simplex virus type 1 is described. It is demonstrated that over the range 10 kHz-20 MHz, these viral particles, when suspended in an aqueous medium of conductivity 5 mS m^-1, can be manipulated by both positive and negative dielectrophoresis using microfabricated electrode arrays. The observed transition from positive to negative dielectrophoresis at frequencies around 4.5 MHz is in qualitative agreement with a simple model of the virus as a conducting particle surrounded by an insulating membrane. Copyright (C) 1998 Elsevier Science B.V.

Original language	British English
Pages (from-to)	119-126
Number of pages	8
Journal	BBA - General Subjects
Volume	1425
Issue number	1
DOIs	https://doi.org/10.1016/S0304-4165(98)00058-0
State	Published - 16 Sep 1998

Keywords

AC electrokinetics
Dielectric property
Nanotechnology
Particle manipulation

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10.1016/S0304-4165(98)00058-0

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title = "Manipulation of herpes simplex virus type 1 by dielectrophoresis",

abstract = "The frequency-dependent dielectrophoretic behaviour of an enveloped mammalian virus, herpes simplex virus type 1 is described. It is demonstrated that over the range 10 kHz-20 MHz, these viral particles, when suspended in an aqueous medium of conductivity 5 mS m-1, can be manipulated by both positive and negative dielectrophoresis using microfabricated electrode arrays. The observed transition from positive to negative dielectrophoresis at frequencies around 4.5 MHz is in qualitative agreement with a simple model of the virus as a conducting particle surrounded by an insulating membrane. Copyright (C) 1998 Elsevier Science B.V.",

keywords = "AC electrokinetics, Dielectric property, Nanotechnology, Particle manipulation",

author = "Hughes, {Michael P.} and Hywel Morgan and Rixon, {Frazer J.} and Burt, {Julian P.H.} and Ronald Pethig",

note = "Funding Information: The authors would like to thank Mrs. Joyce Mitchell and Ms. Mary Robertson for virus preparation, Mr. William Monaghan for electrode fabrication and Mr. Nicolas Green for valuable discussions. This work is supported by the Biotechnology and Biological Sciences Research Council (UK) grant no. 17/T05315. ",

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doi = "10.1016/S0304-4165(98)00058-0",

language = "British English",

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AU - Hughes, Michael P.

AU - Morgan, Hywel

AU - Rixon, Frazer J.

AU - Burt, Julian P.H.

AU - Pethig, Ronald

N1 - Funding Information: The authors would like to thank Mrs. Joyce Mitchell and Ms. Mary Robertson for virus preparation, Mr. William Monaghan for electrode fabrication and Mr. Nicolas Green for valuable discussions. This work is supported by the Biotechnology and Biological Sciences Research Council (UK) grant no. 17/T05315.

PY - 1998/9/16

Y1 - 1998/9/16

N2 - The frequency-dependent dielectrophoretic behaviour of an enveloped mammalian virus, herpes simplex virus type 1 is described. It is demonstrated that over the range 10 kHz-20 MHz, these viral particles, when suspended in an aqueous medium of conductivity 5 mS m-1, can be manipulated by both positive and negative dielectrophoresis using microfabricated electrode arrays. The observed transition from positive to negative dielectrophoresis at frequencies around 4.5 MHz is in qualitative agreement with a simple model of the virus as a conducting particle surrounded by an insulating membrane. Copyright (C) 1998 Elsevier Science B.V.

AB - The frequency-dependent dielectrophoretic behaviour of an enveloped mammalian virus, herpes simplex virus type 1 is described. It is demonstrated that over the range 10 kHz-20 MHz, these viral particles, when suspended in an aqueous medium of conductivity 5 mS m-1, can be manipulated by both positive and negative dielectrophoresis using microfabricated electrode arrays. The observed transition from positive to negative dielectrophoresis at frequencies around 4.5 MHz is in qualitative agreement with a simple model of the virus as a conducting particle surrounded by an insulating membrane. Copyright (C) 1998 Elsevier Science B.V.

KW - AC electrokinetics

KW - Dielectric property

KW - Nanotechnology

KW - Particle manipulation

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SP - 119

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JO - BBA - General Subjects

JF - BBA - General Subjects

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Manipulation of herpes simplex virus type 1 by dielectrophoresis

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Keywords

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