Liquid biphasic system: A recent bioseparation technology

Kuan Shiong Khoo, Hui Yi Leong, Kit Wayne Chew, Jun Wei Lim, Tau Chuan Ling, Pau Loke Show, Hong Wei Yen

Research output: Contribution to journalArticlepeer-review

54 Scopus citations


A well-known bioseparation technique namely liquid biphasic system (LBS) has attracted many researchers' interest for being an alternative bioseparation technology for various kinds of biomolecules. The present review begins with an in-depth discussion on the fundamental principle of LBS and this is followed by the discussion on further development of various phase-forming components in LBS. Additionally, the implementation of various advance technologies to the LBS that is beneficial towards the efficiency of LBS for the extraction, separation, and purification of biomolecules was discussed. The key parameters affecting the LBS were presented and evaluated. Moreover, future prospect and challenges were highlighted to be a useful guide for future development of LBS. The efforts presented in this review will provide an insight for future researches in liquid-liquid separation techniques.

Original languageBritish English
Article number149
Issue number2
StatePublished - 1 Feb 2020


  • Aqueous biphasic system
  • Aqueous two-phase system
  • Biomolecules
  • Liquid biphasic system
  • Purification
  • Recovery
  • Separation


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