Liquid biphasic flotation for the purification of C-phycocyanin from Spirulina platensis microalga

Kit Wayne Chew; Shir Reen Chia; Rambabu Krishnamoorthy; Yang Tao; Dinh Toi Chu; Pau Loke Show

doi:10.1016/j.biortech.2019.121519

Liquid biphasic flotation for the purification of C-phycocyanin from Spirulina platensis microalga

Kit Wayne Chew, Shir Reen Chia, Rambabu Krishnamoorthy, Yang Tao, Dinh Toi Chu, Pau Loke Show

Chemical and Petroleum Engineering

Research output: Contribution to journal › Article › peer-review

78 Scopus citations

Abstract

Liquid biphasic flotation (LBF), an integrated process of liquid biphasic system (LBS) and adsorptive bubbles flotation, was used for the purification of C-phycocyanin from S. platensis microalgae. Various experimental parameters such as type of phase forming polymer and salt, concentration of phase forming components, system pH, volume ratio, air flotation time and crude extract concentration were evaluated to maximise the C-phycocyanin recovery yield and purity. The optimal conditions for the LBF system achieving C-phycocyanin purification fold of 3.49 compared to 2.43 from the initial LBF conditions was in polyethylene glycol (PEG) 4000 and potassium phosphate combination, with 250 g/L of polymer and salt concentration each, volume ratio of 1:0.85, system pH of 7.0, air flotation duration of 7 min and phycocyanin crude extract concentration of 0.625 %w/w. The LBF has effectively enhanced the purification of C-phycocyanin in a cost effective and simple processing.

Original language	British English
Article number	121519
Journal	Bioresource Technology
Volume	288
DOIs	https://doi.org/10.1016/j.biortech.2019.121519
State	Published - Sep 2019

Keywords

C-phycocyanin
Liquid biphasic flotation
Pigment
Purification
Spirulina platensis

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title = "Liquid biphasic flotation for the purification of C-phycocyanin from Spirulina platensis microalga",

abstract = "Liquid biphasic flotation (LBF), an integrated process of liquid biphasic system (LBS) and adsorptive bubbles flotation, was used for the purification of C-phycocyanin from S. platensis microalgae. Various experimental parameters such as type of phase forming polymer and salt, concentration of phase forming components, system pH, volume ratio, air flotation time and crude extract concentration were evaluated to maximise the C-phycocyanin recovery yield and purity. The optimal conditions for the LBF system achieving C-phycocyanin purification fold of 3.49 compared to 2.43 from the initial LBF conditions was in polyethylene glycol (PEG) 4000 and potassium phosphate combination, with 250 g/L of polymer and salt concentration each, volume ratio of 1:0.85, system pH of 7.0, air flotation duration of 7 min and phycocyanin crude extract concentration of 0.625 %w/w. The LBF has effectively enhanced the purification of C-phycocyanin in a cost effective and simple processing.",

keywords = "C-phycocyanin, Liquid biphasic flotation, Pigment, Purification, Spirulina platensis",

author = "Chew, {Kit Wayne} and Chia, {Shir Reen} and Rambabu Krishnamoorthy and Yang Tao and Chu, {Dinh Toi} and Show, {Pau Loke}",

note = "Funding Information: This work was supported by the Fundamental Research Grant Scheme, Malaysia [ FRGS/1/2015/SG05/UNIM/03/1 ]; the Ministry of Science and Technology, Malaysia [ MOSTI02-02-12-SF0256 ]; the Prototype Research Grant Scheme, Malaysia [ PRGS/2/2015/SG05/UNIM/03/1 ]; the International Cooperation Seeds Funding of Nanjing Agricultural University [ 2018-AH-04 ]. Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

year = "2019",

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doi = "10.1016/j.biortech.2019.121519",

language = "British English",

volume = "288",

journal = "Bioresource Technology",

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AU - Chew, Kit Wayne

AU - Chia, Shir Reen

AU - Krishnamoorthy, Rambabu

AU - Tao, Yang

AU - Chu, Dinh Toi

AU - Show, Pau Loke

N1 - Funding Information: This work was supported by the Fundamental Research Grant Scheme, Malaysia [ FRGS/1/2015/SG05/UNIM/03/1 ]; the Ministry of Science and Technology, Malaysia [ MOSTI02-02-12-SF0256 ]; the Prototype Research Grant Scheme, Malaysia [ PRGS/2/2015/SG05/UNIM/03/1 ]; the International Cooperation Seeds Funding of Nanjing Agricultural University [ 2018-AH-04 ]. Publisher Copyright: © 2019 Elsevier Ltd

PY - 2019/9

Y1 - 2019/9

N2 - Liquid biphasic flotation (LBF), an integrated process of liquid biphasic system (LBS) and adsorptive bubbles flotation, was used for the purification of C-phycocyanin from S. platensis microalgae. Various experimental parameters such as type of phase forming polymer and salt, concentration of phase forming components, system pH, volume ratio, air flotation time and crude extract concentration were evaluated to maximise the C-phycocyanin recovery yield and purity. The optimal conditions for the LBF system achieving C-phycocyanin purification fold of 3.49 compared to 2.43 from the initial LBF conditions was in polyethylene glycol (PEG) 4000 and potassium phosphate combination, with 250 g/L of polymer and salt concentration each, volume ratio of 1:0.85, system pH of 7.0, air flotation duration of 7 min and phycocyanin crude extract concentration of 0.625 %w/w. The LBF has effectively enhanced the purification of C-phycocyanin in a cost effective and simple processing.

AB - Liquid biphasic flotation (LBF), an integrated process of liquid biphasic system (LBS) and adsorptive bubbles flotation, was used for the purification of C-phycocyanin from S. platensis microalgae. Various experimental parameters such as type of phase forming polymer and salt, concentration of phase forming components, system pH, volume ratio, air flotation time and crude extract concentration were evaluated to maximise the C-phycocyanin recovery yield and purity. The optimal conditions for the LBF system achieving C-phycocyanin purification fold of 3.49 compared to 2.43 from the initial LBF conditions was in polyethylene glycol (PEG) 4000 and potassium phosphate combination, with 250 g/L of polymer and salt concentration each, volume ratio of 1:0.85, system pH of 7.0, air flotation duration of 7 min and phycocyanin crude extract concentration of 0.625 %w/w. The LBF has effectively enhanced the purification of C-phycocyanin in a cost effective and simple processing.

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KW - Liquid biphasic flotation

KW - Pigment

KW - Purification

KW - Spirulina platensis

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Liquid biphasic flotation for the purification of C-phycocyanin from Spirulina platensis microalga

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Keywords

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