Isolation of Spirosoma foliorum sp. nov. from the fallen leaf of Acer palmatum by a novel cultivation technique: Scientific Reports

H.L. Han, D.A. Nurcahyanto, N. Muhammad, Y.-J. Lee, T.T.H. Nguyen, S.-G. Kim, S.S. Chan, K.S. Khoo, K.W. Chew, P.L. Show, T.N.T. Tran, T.D.P. Nguyen, C.Y. Chiu

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

In the effort of isolating novel microbial species, the strain PL0132T was isolated from a fallen leaf under fresh water at a stream, which glided when grown on a tap water medium (without nutrients). The strain was determined to be Gram-negative, strictly aerobic, and rod-shaped, which grew optimally at 25 °C, pH 6–7, and the strain tolerates 1% (w/v) NaCl concentration. The complete genome of strain PL0132T comprises one contig with a sequencing depth of 76×, consisting of 8,853,064 base pairs and the genomic DNA G + C content was 46.7% (genome). 16S rRNA gene sequence analysis revealed that strain PL0132T represents a member of the phylum Bacteroidetes and is affiliated with the genus Spirosoma. Based on genomic, phenotypic, and chemotaxonomic characteristics, the strain PL0132T represents a novel species of the genus Spirosoma, for which the name Spirosoma foliorum sp. nov. is proposed (= KCTC 72228 T = InaCC B1447T). © 2023, Springer Nature Limited.
Original languageBritish English
JournalSci. Rep.
Volume13
Issue number1
DOIs
StatePublished - 2023

Keywords

  • Acer
  • Bacteroidetes
  • Cytophagaceae
  • Plant Leaves
  • RNA, Ribosomal, 16S
  • RNA 16S
  • Flexibacteraceae
  • genetics
  • plant leaf

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